A colorimetric and ratiometric fluorescent probe for the imaging of endogenous hydrogen sulphide in living cells and sulphide determination in mouse hippocampus.

Abstract	A naphthalimide-azide based colorimetric and ratiometric fluorescent probe, NAP-1, has been developed for the selective and sensitive detection of hydrogen sulphide. Advantages of the probe NAP-1 include a low detection limit (110 nM), good selectivity, high sensitivity and excellent photostability. A linear relationship between the emission intensity ratios and sulphide concentrations was observed in PBS buffer and bovine serum, respectively. Our probe facilitates ratiometric determination and imaging of endogenous H2S in living cells. Furthermore, this probe was successfully applied to the measurement of endogenous sulphide in human plasma and mouse hippocampus. A significant reduction in sulphide levels and CBS mRNA expression was observed in the hippocampus of mouse models of lipopolysaccharide-induced neuroinflammation-related diseases, suggesting that decreased levels of endogenous H2S might be involved in the pathogenesis of neuroinflammation-related neurodegenerative diseases.
Authors	Ling Zhang, Sai Li, Mei Hong, Yuqing Xu, Shuaishuai Wang, Yi Liu, Yong Qian, Jing Zhao
Journal	Organic & biomolecular chemistry (Org Biomol Chem) Vol. 12 Issue 28 Pg. 5115-25 (Jul 28 2014) ISSN: 1477-0539 [Electronic] England
PMID	24874797 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Azides Fluorescent Dyes Lipopolysaccharides Naphthalimides RNA, Messenger Sulfides Cystathionine beta-Synthase sodium bisulfide Hydrogen Sulfide
Topics	Animals Azides (chemical synthesis) Brain (metabolism, pathology) Cattle Colorimetry (methods) Cystathionine beta-Synthase (genetics, metabolism) Fluorescent Dyes (chemical synthesis) Gene Expression Hippocampus Humans Hydrogen Sulfide (analysis, blood, metabolism) Inflammation (chemically induced, diagnosis, metabolism, pathology) Injections, Intraventricular Limit of Detection Lipopolysaccharides MCF-7 Cells Male Mice Molecular Imaging (methods) Naphthalimides (chemical synthesis) RNA, Messenger (genetics, metabolism) Sulfides (administration & dosage)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!