Development of oxacillin resistance in a patient with recurrent Staphylococcus aureus bacteremia.

Abstract

Whole-genome sequencing was used to compare longitudinal isolates of Staphylococcus aureus that developed resistance to oxacillin (MIC up to 16 μg/ml). The mecA gene was absent. A novel 5-bp TATCC frameshift insertion in a gene encoding an ABC transporter similar to that of the teichoic acid translocation ATP-binding protein TagH and a 3-bp GCT nonframeshift insertion in the pdhA pyruvate dehydrogenase gene were detected in the oxacillin-resistant isolates.

• Authors: Eileen M Burd, Mohammad Tauqeer Alam, Karla D Passalacqua, Ameeta S Kalokhe, Molly E Eaton, Sarah W Satola, Colleen S Kraft, Timothy D Read
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 52 Issue 8 Pg. 3114-7 (Aug 2014) ISSN: 1098-660X [Electronic] United States
• PMID: 24850355 (Publication Type: Case Reports, Journal Article)
• Copyright: Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Chemical References

• ATP-Binding Cassette Transporters
• Anti-Bacterial Agents
• DNA, Bacterial
• Oxacillin

Topics

• ATP-Binding Cassette Transporters (genetics)
• Aged, 80 and over
• Anti-Bacterial Agents (pharmacology)
• Bacteremia (microbiology)
• DNA, Bacterial (chemistry, genetics)
• Drug Resistance, Bacterial
• Frameshift Mutation
• Genome, Bacterial
• Humans
• Longitudinal Studies
• Male
• Microbial Sensitivity Tests
• Molecular Sequence Data
• Mutagenesis, Insertional
• Oxacillin (pharmacology)
• Recurrence
• Sequence Analysis, DNA
• Staphylococcal Infections (microbiology)
• Staphylococcus aureus (drug effects, isolation & purification)