Glycine propionyl l-carnitine attenuates d-Galactosamine induced fulminant hepatic failure in wistar rats.

Abstract

Glycine propionyl l-carnitine (GPLC) is a propionyl ester of carnitine that includes an additional glycine component. The present study evaluated hepatoprotective effect of GPLC in d-Galactosamine (d-GalN) induced fulminant hepatic failure. Rats were intraperitonially administered d-GalN (700mg/kgBW). GPLC was given as a pre-treatment (35mg/kgBW/day) for 1month followed by a single dose of d-GalN on the 31st day. d-GalN administration resulted in increased mortality and serum ALT and AST activities. These increases were significantly attenuated by GPLC. d-GalN treatment increased hepatic lipid peroxidation and a decrease in reduced glutathione content was observed. GPLC pre-treatment significantly decreased lipid peroxidation and augmented the level of GSH. d-GalN increased the circulating level of TNF-α and ATM-Kinase and MAP-Kinase expression. GPLC supplementation inhibited the increase in serum TNF-α and ATM-Kinase and MAP-Kinase expression. d-GalN treatment increased the level of Bax and Caspase-3 m-RNA while as a decline was observed in Bcl2 m-RNA. GPLC prevented the increase in Caspase-3 and Bax m-RNA and at the same time augmented the expression of Bcl2 m-RNA. Our findings suggest that GPLC alleviates d-GalN induced liver injury by strengthening antioxidative defense system and reducing apoptotic signalling pathways.

• Authors: Ajaz A Ganai, Sadaf Jahan, Amjid Ahad, M Z Abdin, Humaira Farooqi
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 214 Pg. 33-40 (May 05 2014) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 24565947 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• DNA Primers
• Tumor Necrosis Factor-alpha
• Galactosamine
• Aspartate Aminotransferases
• Alanine Transaminase
• Ataxia Telangiectasia Mutated Proteins
• Mitogen-Activated Protein Kinases
• Glutathione
• glycine propionyl carnitine
• Carnitine
• Glycine

Topics

• Alanine Transaminase (blood)
• Animals
• Aspartate Aminotransferases (blood)
• Ataxia Telangiectasia Mutated Proteins (metabolism)
• Base Sequence
• Carnitine (analogs & derivatives, pharmacology)
• Comet Assay
• DNA Primers
• Galactosamine (toxicity)
• Glutathione (blood)
• Glycine (analogs & derivatives, pharmacology)
• Lipid Peroxidation
• Liver Failure, Acute (chemically induced, prevention & control)
• Male
• Mitogen-Activated Protein Kinases (metabolism)
• Rats
• Rats, Wistar
• Real-Time Polymerase Chain Reaction
• Tumor Necrosis Factor-alpha (blood)