Citrus peel extract attenuates acute cyanide poisoning-induced seizures and oxidative stress in rats.

Abstract

The primary aimed of this study was to investigate the potential protective effects of methanolic extract of citrus peel (MECP) on acute cyanide (KCN) poisoning-induced seizures and oxidative stress in rats. The intraperitoneal LD50 value of KCN (6.3 mg/Kg bwt), based on 24 hrs mortality, was significantly increased by 9, 52 or 113% by oral administration of MECP (500 mg/Kg bwt) pre-administered for 1, 2 and 3 days, respectively, in rats in a time-dependent manner. Intraperitoneal injection of the sublethal dose of KCN (3 mg/Kg bwt) into rats increased, 24 hrs later, lipid peroxidation (LPO), nitric oxide (NO), glutamate levels and acetylcholinesterase (AChE) activity in hippocampus, striatum and cerebral cortex. KCN also decreased brain glutathione (GSH) level and superoxide dismutase (SOD) and catalase (CAT) activities in these animals. Pre-treatment of rats with MECP inhibited KCN-induced increases in LPO, NO, and glutamate levels and AChE activity as well as decreases in brain GSH level and SOD and CAT activities. In addition, KCN significantly decreased norepinephrine, dopamine and serotonin levels in different brain regions which were resolved by MECP. From the present results, it can be concluded that the neuroprotective effects of MECP against KCN-induced seizures and oxidative stress may be due to the inhibition of oxidative stress overproduction and maintenance of antioxidant defense mechanisms.

• Authors: Ahmed E Abdel Moneim
• Journal: CNS & neurological disorders drug targets (CNS Neurol Disord Drug Targets) Vol. 13 Issue 4 Pg. 638-46 ( 2014) ISSN: 1996-3181 [Electronic] United Arab Emirates
• PMID: 24308563 (Publication Type: Journal Article)

Chemical References

• Neuroprotective Agents
• Plant Extracts
• Serotonin Plasma Membrane Transport Proteins
• Nitric Oxide
• Glutamic Acid
• Acetylcholinesterase
• Potassium Cyanide
• Dopamine
• Norepinephrine

Topics

• Acetylcholinesterase (metabolism)
• Animals
• Cerebral Cortex (drug effects, pathology, physiopathology)
• Citrus sinensis
• Corpus Striatum (drug effects, pathology, physiopathology)
• Dopamine (metabolism)
• Fruit
• Glutamic Acid (metabolism)
• Hippocampus (drug effects, pathology, physiopathology)
• Lipid Peroxidation (drug effects, physiology)
• Male
• Neuroprotective Agents (pharmacology)
• Nitric Oxide (metabolism)
• Norepinephrine (metabolism)
• Oxidative Stress (drug effects, physiology)
• Phytotherapy
• Plant Extracts (therapeutic use)
• Potassium Cyanide (poisoning)
• Rats, Wistar
• Seizures (chemically induced, drug therapy, pathology, physiopathology)
• Serotonin Plasma Membrane Transport Proteins (metabolism)