Abstract | OBJECTIVE: METHODS: One hundred seventy healthy rats (85 male and 85 female) were exposed by inhalation of 20,135 and 250 mg/m3 nickel carbonyl for 30 min. Rats poisoned by chlorine gas with a concentration 250 mg/m3 served as positive group and healthy SD rats served as no-treatment negative group. The rats were euthanized on 1, 2, 3 and 7 d after the administration of nickel carbonyl or chlorine gas. In various treatment groups, Na(+)-K+ ATPase activity was studied by colorimetric method and the expressions of Na(+)-K+ ATPase alpha1 mRNA were determined by RT-PCR. RESULTS: Na(+)-K+ ATPase activity and expressions of Na(+)-K+ ATPase alpha1 mRNA in lung tissues decreased in all treatment groups and chlorine gas-poisoned group, especially it was obvious decreased on the 2ed and 3rd day (P < 0.05). CONCLUSION:
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Authors | Haiying Shi, Ning Wang, Qiuying Wang, Hongquan Pu, Xiaopei Zhang, Hui Shang, Xiaoqiang Xuan, Guoyu Ma, Ning Cheng |
Journal | Wei sheng yan jiu = Journal of hygiene research
(Wei Sheng Yan Jiu)
Vol. 42
Issue 5
Pg. 818-21
(Sep 2013)
ISSN: 1000-8020 [Print] China |
PMID | 24218892
(Publication Type: Journal Article)
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Chemical References |
- Organometallic Compounds
- RNA, Messenger
- nickel carbonyl
- Chlorine
- Atp1a1 protein, rat
- Sodium-Potassium-Exchanging ATPase
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Topics |
- Animals
- Chlorine
(poisoning)
- Female
- Lung
(enzymology, pathology)
- Lung Injury
(chemically induced)
- Male
- Organometallic Compounds
(poisoning)
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Sodium-Potassium-Exchanging ATPase
(genetics, metabolism)
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