Abstract |
Neuroinflammation has been considered to be an integrated part of human neurodegenerative diseases. In this study, we examined the effect of guggulipid on cell proliferation, nitrite release, interleukin IL-6 and IL-1 beta release, and expression of COX-2 and glial fibrillary acidic protein (GFAP) in LPS-stimulated U373MG cells. LPS significantly stimulated human astrocytoma cells U373MG by up-regulating these neuroinflammatory mediators. Guggulipid alone had no effect on the cell proliferation of U373MG cells. The up regulation in nitrite release, cell proliferation, and release of IL-6 and IL-1 beta in LPS stimulated human astrocytoma cells were dose-dependently inhibited by co-treatment with guggulipid. The expression level of COX-2 and GFAP proteins was up regulated by LPS but the increased level of COX-2 and GFAP was significantly down regulated by treatment with guggulipid. These data indicate that guggulipid has a modulatory effect on all these parameters, which might explain its beneficial effect in the treatment of neuroinflammation-associated disorders directly relating to human aspects.
|
Authors | Rituraj Niranjan, Rajasekar Nagarajan, Kashif Hanif, Chandishwar Nath, Rakesh Shukla |
Journal | Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
(Neurol Sci)
Vol. 35
Issue 3
Pg. 409-14
(Mar 2014)
ISSN: 1590-3478 [Electronic] Italy |
PMID | 24013551
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Cytokines
- Glial Fibrillary Acidic Protein
- Lipopolysaccharides
- NF-kappa B
- Nitrites
- Plant Extracts
- Plant Gums
- guggulu extract
- Cyclooxygenase 2
- PTGS2 protein, human
|
Topics |
- Astrocytoma
(pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Commiphora
- Cyclooxygenase 2
(metabolism)
- Cytokines
(metabolism)
- Dose-Response Relationship, Drug
- Drug Interactions
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glial Fibrillary Acidic Protein
(metabolism)
- Humans
- Lipopolysaccharides
(pharmacology)
- NF-kappa B
(metabolism)
- Nitrites
(metabolism)
- Plant Extracts
(pharmacology)
- Plant Gums
(pharmacology)
- Protein Transport
(drug effects)
|