Assessing intrarenal nonperfusion and vascular leakage in acute kidney injury with multinuclear (1) H/(19) F MRI and perfluorocarbon nanoparticles.

Abstract

PURPOSE:

We sought to develop a unique sensor-reporter approach for functional kidney imaging that employs circulating perfluorocarbon nanoparticles and multinuclear (1) H/(19) F MRI.

METHODS:

(19) F spin density weighted and T1 weighted images were used to generate quantitative functional mappings of both healthy and ischemia-reperfusion (acute kidney injury) injured mouse kidneys. (1) H blood-oxygenation-level-dependent (BOLD) MRI was also employed as a supplementary approach to facilitate the comprehensive analysis of renal circulation and its pathological changes in acute kidney injury.

RESULTS:

Heterogeneous blood volume distributions and intrarenal oxygenation gradients were confirmed in healthy kidneys by (19) F MRI. In a mouse model of acute kidney injury, (19) F MRI, in conjunction with blood-oxygenation-level-dependent MRI, sensitively delineated renal vascular damage and recovery. In the cortico-medullary junction region, we observed 25% lower (19) F signal (P < 0.05) and 70% longer (1) H T2* (P < 0.01) in injured kidneys compared with contralateral kidneys at 24 h after initial ischemia-reperfusion injury. We also detected 71% higher (19) F signal (P < 0.01) and 40% lower (1) H T2* (P < 0.05) in the renal medulla region of injured kidneys compared with contralateral uninjured kidneys.

CONCLUSION:

Integrated (1) H/(19) F MRI using perfluorocarbon nanoparticles provides a multiparametric readout of regional perfusion defects in acutely injured kidneys.

• Authors: Lingzhi Hu, Junjie Chen, Xiaoxia Yang, Angana Senpan, John S Allen, Noriko Yanaba, Shelton D Caruthers, Gregory M Lanza, Marc R Hammerman, Samuel A Wickline
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 71 Issue 6 Pg. 2186-96 (Jun 2014) ISSN: 1522-2594 [Electronic] United States
• PMID: 23929727 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Fluorocarbons
• Fluorine
• Oxygen

Topics

• Acute Kidney Injury (pathology)
• Animals
• Blood Volume
• Calibration
• Fluorine
• Fluorocarbons (chemical synthesis)
• Kidney (blood supply)
• Magnetic Resonance Imaging (methods)
• Mice
• Mice, Inbred C57BL
• Nanoparticles
• Oxygen (blood)
• Phantoms, Imaging
• Reperfusion Injury (pathology)