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C-type natriuretic peptide transcriptomic profiling increases in human leukocytes of patients with chronic heart failure as a function of clinical severity.

Abstract
The aim of this study was to evaluate the transcriptomic profiling of C-type natriuretic peptide (CNP) and of its specific receptor, NPR-B in human leukocytes of heart failure (HF) patients as a function of clinical severity, assessing the possible changes with respect to healthy subjects (C). mRNA expression was evaluated by Real-Time PCR and total RNA was extracted from leukocytes of C (n=8) and of HF patients (NYHA I-II, n=7; NYHA III-IV, n=13) with PAXgene Blood RNA Kit. Significantly higher levels of CNP mRNA expression were found in HF patients as a function of clinical severity (C=0.23±0.058, NYHA I-II=0.47±0.18, NYHA III-IV=2.58±0.71, p=0.005 C vs NYHA III-IV, p=0.017 NYHA I-II vs NYHA III-IV) and NPR-B transcript levels resulted down-regulated in HF patients with higher NYHA class (C=2.2±0.61, NYHA I-II=2.76±0.46, NYHA III-IV=0.29±0.13, p=0.001 C vs NYHA III-IV, p<0.0001 NYHA I-II vs NYHA III-IV). A significant negative correlation between CNP and NPR-B mRNA expression (r=0.5, p=0.03) was also observed. These results suggest a co-regulation of NPR-B and CNP expression supporting the relevance of this receptor in human disease characterized by a marked inflammatory/immune component and suggesting the possibility of manipulating inflammation via pharmacological agents selective for this receptor.
AuthorsM Cabiati, L Sabatino, R Caruso, A Verde, C Caselli, T Prescimone, D Giannessi, S Del Ry
JournalPeptides (Peptides) Vol. 47 Pg. 110-4 (Sep 2013) ISSN: 1873-5169 [Electronic] United States
PMID23911666 (Publication Type: Journal Article)
CopyrightCopyright © 2013 Elsevier Inc. All rights reserved.
Chemical References
  • RNA, Messenger
  • Natriuretic Peptide, C-Type
  • Receptors, Atrial Natriuretic Factor
  • atrial natriuretic factor receptor B
Topics
  • Adult
  • Chronic Disease
  • Female
  • Gene Expression Profiling
  • Heart Failure (blood, genetics, physiopathology)
  • Humans
  • Leukocytes, Mononuclear (metabolism)
  • Male
  • Middle Aged
  • Natriuretic Peptide, C-Type (genetics, metabolism)
  • RNA, Messenger (genetics, metabolism)
  • Receptors, Atrial Natriuretic Factor (genetics, metabolism)
  • Severity of Illness Index

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