Abstract |
The aim of this study was to evaluate the transcriptomic profiling of C-type natriuretic peptide (CNP) and of its specific receptor, NPR-B in human leukocytes of heart failure (HF) patients as a function of clinical severity, assessing the possible changes with respect to healthy subjects (C). mRNA expression was evaluated by Real-Time PCR and total RNA was extracted from leukocytes of C (n=8) and of HF patients (NYHA I-II, n=7; NYHA III-IV, n=13) with PAXgene Blood RNA Kit. Significantly higher levels of CNP mRNA expression were found in HF patients as a function of clinical severity (C=0.23±0.058, NYHA I-II=0.47±0.18, NYHA III-IV=2.58±0.71, p=0.005 C vs NYHA III-IV, p=0.017 NYHA I-II vs NYHA III-IV) and NPR-B transcript levels resulted down-regulated in HF patients with higher NYHA class (C=2.2±0.61, NYHA I-II=2.76±0.46, NYHA III-IV=0.29±0.13, p=0.001 C vs NYHA III-IV, p<0.0001 NYHA I-II vs NYHA III-IV). A significant negative correlation between CNP and NPR-B mRNA expression (r=0.5, p=0.03) was also observed. These results suggest a co-regulation of NPR-B and CNP expression supporting the relevance of this receptor in human disease characterized by a marked inflammatory/immune component and suggesting the possibility of manipulating inflammation via pharmacological agents selective for this receptor.
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Authors | M Cabiati, L Sabatino, R Caruso, A Verde, C Caselli, T Prescimone, D Giannessi, S Del Ry |
Journal | Peptides
(Peptides)
Vol. 47
Pg. 110-4
(Sep 2013)
ISSN: 1873-5169 [Electronic] United States |
PMID | 23911666
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- RNA, Messenger
- Natriuretic Peptide, C-Type
- Receptors, Atrial Natriuretic Factor
- atrial natriuretic factor receptor B
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Topics |
- Adult
- Chronic Disease
- Female
- Gene Expression Profiling
- Heart Failure
(blood, genetics, physiopathology)
- Humans
- Leukocytes, Mononuclear
(metabolism)
- Male
- Middle Aged
- Natriuretic Peptide, C-Type
(genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Receptors, Atrial Natriuretic Factor
(genetics, metabolism)
- Severity of Illness Index
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