Abstract | OBJECTIVE: DESIGN: In intestinal tissue from patients with IBD and mice with dextran sodium sulfate (DSS)-induced colitis, MMP8, MMP9 and PE were evaluated by ELISA, immunoblot and immunohistochemistry. Peripheral blood polymorphonuclear cell (PMN) supernatants were also analysed accordingly and incubated with collagen to assess PGP generation ex vivo. PGP levels were measured by mass spectrometry, and PGP neutralisation was achieved with a PGP antagonist and PGP antibodies. RESULTS: In the intestine of patients with IBD, MMP8 and MMP9 levels were elevated, while PE was expressed at similar levels to control tissue. PGP levels were increased in intestinal tissue of patients with IBD. Similar results were obtained in intestine from DSS-treated mice. PMN supernatants from patients with IBD were far more capable of generating PGP from collagen ex vivo than healthy controls. Furthermore, PGP neutralisation during DSS-induced colitis led to a significant reduction in neutrophil infiltration in the intestine. CONCLUSIONS: The proteolytic cascade that generates PGP from collagen, as well as the tripeptide itself, is present in the intestine of patients with IBD and mice with DSS-induced colitis. PGP neutralisation in DSS-treated mice showed the importance of PGP-guided neutrophilic infiltration in the intestine and indicates a vicious circle in neutrophilic inflammation in IBD.
|
Authors | Pim J Koelink, Saskia A Overbeek, Saskia Braber, Mary E Morgan, Paul A J Henricks, Mojtaba Abdul Roda, Hein W Verspaget, Simone C Wolfkamp, Anje A te Velde, Caleb W Jones, Patricia L Jackson, J Edwin Blalock, Rolf W Sparidans, John A W Kruijtzer, Johan Garssen, Gert Folkerts, Aletta D Kraneveld |
Journal | Gut
(Gut)
Vol. 63
Issue 4
Pg. 578-87
(Apr 2014)
ISSN: 1468-3288 [Electronic] England |
PMID | 23525573
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Collagen
- Serine Endopeptidases
- PREPL protein, human
- Prolyl Oligopeptidases
- Matrix Metalloproteinase 8
- Matrix Metalloproteinase 9
|
Topics |
- Adolescent
- Adult
- Aged
- Animals
- Child
- Collagen
(metabolism)
- Disease Models, Animal
- Female
- Humans
- Inflammatory Bowel Diseases
(metabolism, physiopathology)
- Intestinal Mucosa
(metabolism)
- Intestines
(enzymology, physiopathology)
- Male
- Matrix Metalloproteinase 8
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Mice
- Mice, Inbred C57BL
- Middle Aged
- Neutrophil Infiltration
(physiology)
- Prolyl Oligopeptidases
- Serine Endopeptidases
(metabolism)
- Young Adult
|