[Mechanism of Novaferon on production of TNF-α by monocytes isolated from normal human peripheral blood].

Abstract

OBJECTIVE:

To study the role of Novaferon on TNF-α production and expression of NF-κB mRNA in monocytes isolated from normal human peripheral blood and to provide theoretical basis for treatment of immunological diseases with Novaferon.

METHODS:

Monocytes were isolated from the peripheral blood in 30 healthy volunteers and divided into 5 groups: group A was blank control, group B was stimulated by LPS without Novaferon intervention, group C by LPS together with Novaferon intervention, group D by LPS before Novaferon intervention, which group E by LPS after Novaferon intervention. We detected the concentration of TNF-α after LPS stimulation and Novaferon intervention in the supernatant by ELISA and expression of NF-κB mRNA by RT-PCR.

RESULTS:

Novaferon inhibited TNF-α production by monocytes isolated from healthy volunteers induced by LPS in vitro in group D compared with group B [(1446.76±72.36) pg/mL vs (946.46±46.12) pg/mL, P<0.01], and the rate was 29.7%. There was no significant change in TNF-α concentration in group C and E compared with group B [(1446.76±72.36) pg/mL vs (1275.62±87.75) pg/mL, P>0.05; (1446.76±72.36) pg/mL vs (1383.62±86.96) pg/mL, P>0.05]. There was significant change in NF-κB mRNA expression in group D compared with group B (0.2829±0.0365 vs 0.4994±0.0604, P<0.01). There was no significant change in NF-κB mRNA expression in group C and group E compared with group B (0.4716±0.0616 vs 0.4994±0.0604, P>0.05; 0.4767±0.0600 vs 0.4994±0.0604, P>0.05).

CONCLUSION:

Novaferon can suppress TNF-α secretion by monocytes induced by LPS in vitro, and it can affect the immunity function of monocytes, which may be associated with the downregulation of NF-κB mRNA expression in monocytes.

• Authors: Fujun Li, Mei Xue, Fanggen Lu, Yiyou Zou
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 38 Issue 1 Pg. 66-9 (Jan 2013) ISSN: 1672-7347 [Print] China
• PMID: 23406858 (Publication Type: English Abstract, Journal Article)

Chemical References

• Lipopolysaccharides
• NF-kappa B
• RNA, Messenger
• Recombinant Proteins
• Tumor Necrosis Factor-alpha

Topics

• Humans
• Lipopolysaccharides (pharmacology)
• Monocytes (drug effects, metabolism)
• NF-kappa B (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Recombinant Proteins (pharmacology)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, metabolism)