Evaluation of amentoflavone isolated from Cnestis ferruginea Vahl ex DC (Connaraceae) on production of inflammatory mediators in LPS stimulated rat astrocytoma cell line (C6) and THP-1 cells.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Cnestisferruginea (CF) Vahl ex DC (Connaraceae) is a shrub widely used in traditional African medicine for the treatment of various psychiatric illness and inflammatory conditions.

AIM OF THE STUDY:

This study was carried out to investigate the effect of amentoflavone isolated from methanolic root extract of CF on lipopolysaccharide (LPS)-induced neuroinflammatory cascade of events associated to the oxidative and nitrative stress, and TNF-α production in rat astrocytoma cell line (C6) and human monocytic leukemia cell line (THP-1), respectively.

MATERIALS AND METHODS:

Rat astrocytoma cells (C6) were stimulated with LPS (10μg/ml) alone and in the presence of different concentrations of amentoflavone (0.1-3μg/ml) for 24h incubation period. Nitrite release, reactive oxygen species (ROS), malondialdehyde (MDA) and reduced-glutathione (GSH) in C6 cells were estimated; while the TNF-α level was estimated in THP-1 cell lysate. In vivo analgesic activity was evaluated using mouse writhing and hot plate tests while the anti-inflammatory effect was investigated using carrageenan-induced oedema test.

RESULTS:

LPS (10μg/ml) significantly (P<0.05) stimulated C6 cells to release nitrite, ROS, MDA, and TNF-α generation while GSH was down regulated in comparison to control. However, amentoflavone significantly (P<0.05) attenuated nitrite, ROS, MDA and TNF-α generation and also up regulated the level of GSH. Amentoflavone per se did not have any significant effect on C6 and THP-1 cells. Amentoflavone (6.25-50mg/kg) significantly (P<0.05) reduced number of writhes and also increase pain threshold in hot plate test. It produced time course significant (P<0.05) decrease in oedema formation in rodents.

DISCUSSION AND CONCLUSION:

Findings in this study demonstrate the anti-neuroinflammatory and antinoceptive effects of amentoflavone which may suggest its beneficial roles in neuroinflammation associated disorders.

• Authors: I O Ishola, J P Chaturvedi, S Rai, N Rajasekar, O O Adeyemi, R Shukla, T Narender
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 146 Issue 2 Pg. 440-8 (Mar 27 2013) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 23376104 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Biflavonoids
• Inflammation Mediators
• Lipopolysaccharides
• Nitrites
• Reactive Oxygen Species
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Carrageenan
• amentoflavone
• Glutathione
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Astrocytoma
• Biflavonoids (pharmacology, therapeutic use)
• Carrageenan
• Cell Line
• Cell Line, Tumor
• Cell Survival
• Connaraceae
• Edema (chemically induced, drug therapy)
• Female
• Glutathione (metabolism)
• Hot Temperature
• Inflammation Mediators (metabolism)
• Lipopolysaccharides
• Male
• Malondialdehyde (metabolism)
• Mice
• Nitrites (metabolism)
• Pain (drug therapy, etiology)
• Phytotherapy
• Plant Roots
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)