Abstract | OBJECTIVE: To compare mitochondrial complex I and complex IV activity in myocardial mitochondria of clinically normal dogs, clinically normal dogs exposed to inhalation anesthesia, and dogs affected with dilated cardiomyopathy. SAMPLE: PROCEDURES: Activity of mitochondrial complex I and complex IV was assayed spectrophotometrically in isolated mitochondria from left ventricular tissue obtained from the 4 groups of dogs. RESULTS: Activity of complex I and complex IV was significantly decreased in anesthetized dogs, compared with activities in the control dogs and dogs with juvenile-onset or adult-onset dilated cardiomyopathy. CONCLUSIONS AND CLINICAL RELEVANCE:
Inhalation anesthesia disrupted the electron transport chain in the dogs, which potentially led to an outburst of reactive oxygen species that caused mitochondrial dysfunction. Inhalation anesthesia depressed mitochondrial function in dogs, similar to results reported in other species. This effect is important to consider when anesthetizing animals with myocardial disease and suggested that antioxidant treatments may be beneficial in some animals. Additionally, this effect should be considered when designing studies in which mitochondrial enzyme activity will be measured. Additional studies that include a larger number of animals are warranted.
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Authors | Meg M Sleeper, Bradley P Rosato, Seema Bansal, Narayan G Avadhani |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 73
Issue 11
Pg. 1759-64
(Nov 2012)
ISSN: 1943-5681 [Electronic] United States |
PMID | 23106461
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Anesthesia, Inhalation
- Anesthetics, Inhalation
- Animals
- Cardiomyopathy, Dilated
(pathology, veterinary)
- Dog Diseases
(pathology)
- Dogs
- Mitochondria, Heart
(metabolism, pathology)
- Myocardium
(metabolism, pathology)
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