Abstract | BACKGROUND/PURPOSE: Drug resistance often causes treatment failure in neuroblastomas. Increasing evidence has implicated that the micro-RNAs ( miRNAs) are involved in the development of drug resistance. In this report, we aimed to investigate the role of miRNA in cisplatin resistance of neuroblastoma cells. MATERIALS AND METHODS: RESULTS: The expression of miR-21 was increased in the cisplatin-resistant (CisR) neuroblastoma cells as compared with the parental cells, and the antagomir against miR-21 converted the resistant cells into sensitive ones. Ectopic expression of pre-miR-21 in parental cells resulted in decreased sensitivity to cisplatin treatment. In addition, overexpression of pre-miR-21 markedly increa sed the proliferation rate of neuroblastoma cells. The level of PTEN messenger RNA and protein in the CisR cells was lower than that in the parental cells. Transfection of pre-miR-21 into the parental cells reduced the PTEN expression, and transfection of anti-miR-21 into the CisR cells increased the PTEN expression. CONCLUSION: Micro-RNA-21 regulated the drug resistance and proliferation in neuroblastoma cells.
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Authors | Yun Chen, Ya-Hui Tsai, Yu Fang, Sheng-Hong Tseng |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 47
Issue 10
Pg. 1797-805
(Oct 2012)
ISSN: 1531-5037 [Electronic] United States |
PMID | 23084187
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- MicroRNAs
- Cisplatin
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Topics |
- Antineoplastic Agents
(therapeutic use)
- Cisplatin
(therapeutic use)
- Drug Resistance, Neoplasm
(genetics)
- Humans
- MicroRNAs
(physiology)
- Neuroblastoma
(drug therapy, genetics)
- Tumor Cells, Cultured
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