Abstract | OBJECTIVE: METHODS: Twenty-four male Wistar rats were divided into 4 groups: sham-operated, torsion/detorsion, torsion/detorsion plus melatonin, and torsion/detorsion plus ozone. Melatonin (10 mg/kg) and ozone (4 mg/kg) were intraperitoneally injected daily beginning 15 minutes before detorsion for the following 7 days. At the seventh day, blood and tissue samples were obtained. Johnsen score, malondialdehyde, inhibin B, glutathione plasma total sulfhydryl group (RSH) levels, and total nitric oxide were studied. RESULTS: Torsion/detorsion caused increase in tissue malondialdehyde and total nitric oxide along with a decrease in Johnsen score, tissue and plasma inhibin B, RSH, and glutathione levels. Melatonin prevented the rise in malondialdehyde and total nitric oxide levels and improved Johnsen score, tissue and plasma inhibin B, and tissue glutathione levels, along with a decrease in plasma RSH level. Ozone showed similar results except for the total nitric oxide level. Concomitantly, in contralateral testis, melatonin and ozone induced similar changes for Johnsen score, malondialdehyde, and inhibin B (not significant) and in glutathione (significant). Melatonin decreased the total nitric oxide level in both testes and ozone increased the same parameter. CONCLUSION:
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Authors | Sinan Ekici, A Işın Doğan Ekici, Güler Öztürk, Fehime Benli Aksungar, Orhun Sinanoğlu, Güven Turan, Nurettin Lüleci |
Journal | Urology
(Urology)
Vol. 80
Issue 4
Pg. 899-906
(Oct 2012)
ISSN: 1527-9995 [Electronic] United States |
PMID | 22950989
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- Oxidants, Photochemical
- inhibin B
- Nitric Oxide
- Malondialdehyde
- Inhibins
- Ozone
- Glutathione
- Melatonin
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Topics |
- Animals
- Antioxidants
(therapeutic use)
- Disease Models, Animal
- Glutathione
(metabolism)
- Inhibins
(metabolism)
- Injections, Intraperitoneal
- Male
- Malondialdehyde
(metabolism)
- Melatonin
(therapeutic use)
- Nitric Oxide
(metabolism)
- Oxidants, Photochemical
(therapeutic use)
- Ozone
(therapeutic use)
- Rats
- Rats, Wistar
- Reperfusion Injury
(etiology, metabolism, prevention & control)
- Spermatic Cord Torsion
(complications, metabolism)
- Statistics, Nonparametric
- Testis
(metabolism, pathology)
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