Abstract | BACKGROUND: Chronic rhinosinusitis (CRS) is an inflammatory upper-airway disease with numerous etiologies. Patients with a characteristic subtype of CRS, allergic fungal rhinosinusitis (AFRS), display increased expression of T helper 2 (Th2) cytokines and antigen-specific immunoglobulin E ( IgE). Various sinonasal inflammatory conditions are associated with alterations in epithelial barrier function. The aim of this study was to compare epithelial permeability and intercellular junctional protein expression among cultured primary sinonasal cells from AFRS patients vs noninflammatory controls. METHODS: Epithelial cells isolated from paranasal sinus mucosa of AFRS and noninflammatory control patients were grown to confluence on permeable supports and transitioned to air-liquid interface (ALI). Transepithelial resistance (TER) was measured with a horizontal Ussing chamber to characterize the functional permeability of each cell type. After TER recordings were complete, a panel of intercellular junctional proteins was assessed by Western blot and immunofluorescence labeling followed by confocal microscopy. RESULTS: CONCLUSION: Cultured sinonasal epithelium from AFRS patients displayed increased epithelial permeability and altered expression of intercellular junctional proteins. Given that these cells were not incubated with inflammatory cytokines in vitro, the cultured AFRS epithelial alterations may represent a retained modification in protein expression from the in vivo phenotype.
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Authors | Kyle A Den Beste, Elizabeth K Hoddeson, Charles A Parkos, Asma Nusrat, Sarah K Wise |
Journal | International forum of allergy & rhinology
(Int Forum Allergy Rhinol)
Vol. 3
Issue 1
Pg. 19-25
(Jan 2013)
ISSN: 2042-6984 [Electronic] United States |
PMID | 22927233
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 American Rhinologic Society-American Academy of Otolaryngic Allergy, LLC. |
Chemical References |
- Claudin-2
- Junctional Adhesion Molecule A
- Occludin
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Topics |
- Blotting, Western
- Claudin-2
(metabolism)
- Epithelial Cells
(metabolism)
- Humans
- Intercellular Junctions
(metabolism)
- Intracellular Membranes
(metabolism)
- Junctional Adhesion Molecule A
(metabolism)
- Microscopy, Confocal
- Occludin
(metabolism)
- Paranasal Sinuses
(metabolism)
- Permeability
- Rhinitis
(metabolism)
- Sinusitis
(metabolism)
- Tight Junctions
(metabolism)
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