Identification of proteins regulated by ferulic acid in a middle cerebral artery occlusion animal model-a proteomics approach.

Abstract

Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD(+)], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes.

• Authors: Jin-Hee Sung, Eun-Hae Cho, Jae-Hyeon Cho, Chung-Kil Won, Myeong-Ok Kim, Phil-Ok Koh
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 74 Issue 11 Pg. 1401-7 (Nov 2012) ISSN: 1347-7439 [Electronic] Japan
• PMID: 22785056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coumaric Acids
• DNA Primers
• Proteins
• ferulic acid
• Isocitrate Dehydrogenase
• Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
• MAP Kinase Kinase 1
• Adenosylhomocysteinase

Topics

• Adenosylhomocysteinase (metabolism)
• Animals
• Blotting, Western
• Brain (metabolism)
• Brain Ischemia (drug therapy, etiology, metabolism)
• Coumaric Acids (pharmacology)
• DNA Primers (genetics)
• Electrophoresis, Gel, Two-Dimensional
• Gene Expression Regulation, Enzymologic (drug effects, genetics)
• Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) (metabolism)
• Infarction, Middle Cerebral Artery (complications)
• Isocitrate Dehydrogenase (metabolism)
• MAP Kinase Kinase 1 (metabolism)
• Male
• Mass Spectrometry
• Polymerase Chain Reaction
• Proteins (isolation & purification, metabolism)
• Proteomics (methods)
• Rats
• Rats, Sprague-Dawley