Peptaibols from two unidentified fungi of the order Hypocreales with cytotoxic, antibiotic, and anthelmintic activities.
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| Abstract |
As part of an ongoing investigation of filamentous fungi for anticancer leads, an active culture was identified from the Mycosynthetix library (MSX 70741, of the order Hypocreales, Ascomycota). The fungal extract exhibited cytotoxic activity against the H460 (human nonsmall cell lung carcinoma) cell line, and bioactivity-directed fractionation yielded peptaibols 1-12 and harzianums A (13) and B (14). Structure elucidation of 1-12 was facilitated by high-resolution MS/MS using higher-energy collisional dissociation and by high field NMR (950 MHz). The absolute configuration was determined by Marfey's analysis of the individual amino acids; the time required for such analysis was decreased via the development of a 10-min ultra performance liquid chromatography method. The isolated peptaibols (1-12), along with three other peptaibols isolated and elucidated from a different fungus (MSX 57715) of the same order (15-17), were examined for activity in a suite of biological assays, including those for cytotoxic, antibacterial, and anthelmintic activities.
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| Authors | Sloan Ayers, Brandie M Ehrmann, Audrey F Adcock, David J Kroll, Esperanza J Carcache de Blanco, Qi Shen, Steven M Swanson, Joseph O Falkinham 3rd, Mansukh C Wani, Sheila M Mitchell, Cedric J Pearce, Nicholas H Oberlies |
| Journal | Journal of peptide science : an official publication of the European Peptide Society (J Pept Sci) Vol. 18 Issue 8 Pg. 500-10 (Aug 2012) ISSN: 1099-1387 [Electronic] England |
| PMID | 22744757 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd. |
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