Evaluation of the cytotoxicity of Satureja spicigera and its main compounds.

Abstract

Satureja spicigera (Lamiaceae) grows wildly in Northwest of Iran. In this study, bioassay-guided isolation and identification of the main compounds has been reported using various chromatographic methods and comparison of their spectral data with those reported in the literature. Brine shrimp lethality and four cancerous cell lines HT29/219, Caco(2), NIH-3T3, and T47D were used for cytotoxicity evaluations. From the aerial parts of S. spicigera, nine known compounds including two flavanones, 5,7,3',5'-tetrahydroxy flavanone (8) and 5,4'-dihydroxy-3'-methoxyflavanone-7-(6''-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside (9), one dihydrochalcone, nubigenol (7), together with thymoquinone (1), thymol (2), carvacrol (3), β-sitosterol (4), ursolic acid (5) and oleanolic acid (6) were identified. Among the isolated chalcone and flavanones, compound 8 was effective against Artemia salina larva (LC(50)= 2 μg/mL) and only the compound 9 demonstrated IC(50) value of 98.7 μg/mL on the T47D (human, breast, ductal carcinoma). Other compounds did not show significant inhibition of the cell growth.

• Authors: Ahmad Reza Gohari, Seyed Nasser Ostad, Fahimeh Moradi-Afrapoli, Maryam Malmir, Shohreh Tavajohi, Hassan Akbari, Soodabeh Saeidnia
• Journal: TheScientificWorldJournal (ScientificWorldJournal) Vol. 2012 Pg. 203861 ( 2012) ISSN: 1537-744X [Electronic] United States
• PMID: 22623883 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Flavanones
• Plant Extracts

Topics

• Animals
• Antineoplastic Agents, Phytogenic (toxicity)
• Artemia (drug effects)
• Caco-2 Cells
• Cell Line, Tumor
• Flavanones (toxicity)
• Flowers (chemistry)
• HT29 Cells
• Humans
• Lethal Dose 50
• Mice
• NIH 3T3 Cells
• Plant Extracts (toxicity)
• Satureja (chemistry)
• Toxicity Tests