Abstract | BACKGROUND: RESULTS: A total of 24 plasma samples were depleted of fourteen high-abundant plasma proteins, analyzed with 2D SDS-PAGE, compared, and statistically processed with Progenesis SameSpots software. Proteins were identified by nanoLC-MS/MS. Retinol-binding protein 4 and leucine-rich alpha-2-glycoprotein were relatively quantified using mass spectrometry. 56 significantly differing spots were found; and in 52 spots 50 different proteins were successfully identified. Several plasma proteins that changed either in their level or modification have been described herein. The plasma level of retinol-binding protein 4 was decreased, while leucine-rich alpha-2-glycoprotein was modified in RAEB-1 patients. Changes in the inter-alpha-trypsin inhibitor heavy chain H4, altered protein fragmentation, or fragments modifications were observed. CONCLUSIONS:
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Authors | Pavel Májek, Zuzana Reicheltová, Jiří Suttnar, Jaroslav Cermák, Jan E Dyr |
Journal | Proteome science
(Proteome Sci)
Vol. 10
Issue 1
Pg. 31
(May 08 2012)
ISSN: 1477-5956 [Electronic] England |
PMID | 22568928
(Publication Type: Journal Article)
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