[Anti-apoptotic role of mitochondrial aldehyde dehydrogenase 2 in myocardial ischemia/reperfusion injury in diabetic rats].

Abstract

OBJECTIVE:

To evaluate the anti-apoptotic effect of aldehyde dehydrogenase 2 (ALDH2) on myocardial ischemia/reperfusion (I/R) injury in diabetic rats.

METHODS:

Normal male SD rats were divided into normal, diabetes and ethanol (the agonist of ALDH2) + diabetes groups. In the latter two groups, diabetes was induced by an intraperitoneal injection of 55 mg/kg STZ. Four weeks after the modeling, myocardial I/R was mimicked ex vivo, and lactate dehydrogenase (LDH) content in the coronary flow was determined. The activities of caspase-3 and ALDH2 were evaluated, and the expressions of Bcl-2 and Bax mRNA in the left anterior myocardium were detected using RT-PCR.

RESULTS:

In diabetic group, LDH release and caspase-3 activity were increased, while ALDH2 activity and Bcl-2/Bax mRNA expression were decreased as compared to those in normal control group. Compared with the diabetic group, ALDH2 agonist ethanol significantly reduced LDH release and caspase-3 activity, increased ALDH2 activity and Bcl-2/Bax mRNA expression.

CONCLUSION:

In diabetic rats, enhanced ALDH2 expression can offer mycardial protection possibly in relation to suppress cell apoptosis.

• Authors: Hong-Ju Wang, Pin-Fang Kang, Hong-Wei Ye, Ying Yu, Xiao-Mei Wang, Qin Gao
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 32 Issue 3 Pg. 345-8 (Mar 2012) ISSN: 1673-4254 [Print] China
• PMID: 22445980 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bax protein, rat
• Mitochondrial Proteins
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• Ethanol
• Aldehyde Dehydrogenase
• Aldehyde Dehydrogenase, Mitochondrial
• Aldh2 protein, rat
• Casp3 protein, rat
• Caspase 3

Topics

• Aldehyde Dehydrogenase (metabolism)
• Aldehyde Dehydrogenase, Mitochondrial
• Animals
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Diabetes Mellitus, Experimental (complications, enzymology)
• Ethanol (pharmacology)
• Male
• Mitochondrial Proteins (agonists, metabolism)
• Myocardial Ischemia (enzymology, etiology)
• Myocardial Reperfusion Injury (enzymology, pathology, prevention & control)
• Myocardium (enzymology, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Rats
• Rats, Sprague-Dawley
• bcl-2-Associated X Protein (metabolism)