Identification of a common immune signature in murine and human systemic Salmonellosis.

Abstract

Despite the importance of Salmonella infections in human and animal health, the target antigens of Salmonella-specific immunity remain poorly defined. We have previously shown evidence for antibody-mediating protection against invasive Salmonellosis in mice and African children. To generate an overview of antibody targeting in systemic Salmonellosis, a Salmonella proteomic array containing over 2,700 proteins was constructed and probed with immune sera from Salmonella-infected mice and humans. Analysis of multiple inbred mouse strains identified 117 antigens recognized by systemic antibody responses in murine Salmonellosis. Importantly, many of these antigens were independently identified as target antigens using sera from Malawian children with Salmonella bacteremia, validating the study of the murine model. Furthermore, vaccination with SseB, the most prominent antigenic target in Malawian children, provided mice with significant protection against Salmonella infection. Together, these data uncover an overlapping immune signature of disseminated Salmonellosis in mice and humans and provide a foundation for the generation of a protective subunit vaccine.

• Authors: Seung-Joo Lee, Li Liang, Silvia Juarez, Minelva R Nanton, Esther N Gondwe, Chisomo L Msefula, Matthew A Kayala, Francesca Necchi, Jennifer N Heath, Peter Hart, Renée M Tsolis, Robert S Heyderman, Calman A MacLennan, Philip L Felgner, D Huw Davies, Stephen J McSorley
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 109 Issue 13 Pg. 4998-5003 (Mar 27 2012) ISSN: 1091-6490 [Electronic] United States
• PMID: 22331879 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Vaccines, Attenuated
• Vaccines, Subunit

Topics

• Animals
• Antibody Formation (immunology)
• Antigens, Bacterial (immunology)
• Bacterial Proteins (immunology)
• Blood Bactericidal Activity
• Child
• Child, Preschool
• Convalescence
• Female
• Humans
• Infant
• Infant, Newborn
• Malawi
• Male
• Mice
• Mice, Inbred Strains
• Protein Array Analysis
• Reproducibility of Results
• Salmonella Infections (blood, immunology)
• Salmonella Infections, Animal (immunology)
• Vaccination
• Vaccines, Attenuated (immunology)
• Vaccines, Subunit (immunology)