Neuroprotective effects of scutellarin against hypoxic-ischemic-induced cerebral injury via augmentation of antioxidant defense capacity.

Abstract

An increasing number of studies has indicated that hypoxic-ischemic-induced cerebral injury is partly mediated via oxidative stress. Recent researches have focused on searching for drug and herbal manipulations to protect against hypoxic-ischemic-induced oxidative cell damage. Scutellarin is a flavonoid derived from the Erigeron breviscapus (vant.) and has been reported to exhibit neuroprotective properties. However, its precise mechanism, particularly its antioxidation mechanism, remains elusive. In the present study, we investigated the neuroprotective effects of scutellarin on middle cerebral artery occlusion (MCAO)-induced brain damage in rats, and oxygen-glucose deprivation (OGD)-induced toxicity in primary culture of rat cortical neurons. In vivo, intraperitoneal injections of scutellarin (20 and 60 mg/kg) improved the neurological score and diminished the percentage of brain infarct volume. At the same time, scutellarin significantly increased superoxide dismutase (SOD), catalase (CAT) activities and glutathione (GSH) level in ischemic brain tissues, enhancing endogenous antioxidant activity. Moreover, pretreatment of scutellarin (25, 50 and 100 μM) protected neurons against lethal stimuli, decreased the percentage of apoptotic cells and inhibited reactive oxygen species (ROS) generation in OGD-induced primary cortical neurons in vitro. These results suggest that the preventive and therapeutic potential of scutellarin in cerebral injury patients is, at least in part, ascribed to augmentation of cellular antioxidant defense capacity.

• Authors: Hong Guo, Li-Min Hu, Shao-Xia Wang, Yu-Lin Wang, Fang Shi, Hui Li, Yang Liu, Li-Yuan Kang, Xiu-Mei Gao
• Journal: The Chinese journal of physiology (Chin J Physiol) Vol. 54 Issue 6 Pg. 399-405 (Dec 31 2011) ISSN: 0304-4920 [Print] India
• PMID: 22229507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Glucuronates
• Neuroprotective Agents
• Reactive Oxygen Species
• scutellarin
• Apigenin

Topics

• Animals
• Antioxidants (metabolism)
• Apigenin (chemistry, pharmacology)
• Brain Infarction (drug therapy, metabolism)
• Cerebral Cortex (cytology)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Glucuronates (chemistry, pharmacology)
• Hypoxia-Ischemia, Brain (drug therapy, metabolism)
• Infarction, Middle Cerebral Artery (drug therapy, metabolism)
• Male
• Neurons (cytology, drug effects, metabolism)
• Neuroprotective Agents (chemistry, pharmacology)
• Oxidative Stress (drug effects)
• Primary Cell Culture
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)