Antioxidant capacities and total polyphenol contents of hydro-ethanolic extract of phytococktail from trans-Himalaya.
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| Abstract |
The aim of this study was to evaluate the in vitro antioxidant potential of hydro-ethanolic extract of a novel phytococktail comprising of sea buckthorn, apricot, and Rhodiola (SAR) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) activity of the extract increased in a dose-dependent manner (upto 0.1 mg/mL), and was found to be about 38% of that of ascorbic acid at 0.1 mg/mL. The hydro-ethanolic extract of SAR also scavenged the ABTS(.+) radical generated by ABTS/potassium persulfate (PPS) system and was found to be about 62% of that of ascorbic acid at 0.1 mg/ mL. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total phenolic content was found to be 1.28016 × 10(-3) mol gallic acid equivalent (GAE)/g extract. Total flavonoid and flavonol contents were estimated to be 2.5970 × 10(-4) mol and 4.87 × 10(-4) mol quercetin equivalent/g extract, respectively. The hydro-ethanolic extract of this phytococktail indicated presence of essential phytoconstituents of polyphenols, flavonoids, flavonols, and ascorbic acid, which contributed significantly to its antioxidant capacity. The combination of the 3 plants may well support their use in traditional medicine to combat oxidative stress and high-altitude sickness.
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| Authors | P Dhar, A B Tayade, P K Bajpai, V K Sharma, S K Das, O P Chaurasia, R B Srivastava, S B Singh |
| Journal | Journal of food science (J Food Sci) Vol. 77 Issue 2 Pg. C156-61 (Feb 2012) ISSN: 1750-3841 [Electronic] United States |
| PMID | 22225422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2012 DRDO Journal of Food Science © 2012 Institute of Food Technologists® |
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