Semi-solid topical formulations containing nimesulide-loaded nanocapsules showed in-vivo anti-inflammatory activity in chronic arthritis and fibrovascular tissue models.

Abstract

OBJECTIVE:

To evaluate the anti-inflammatory activity of topical gel containing nimesulide-loaded nanocapsules using experimental models in vivo, and to compare the anti-inflammatory activity of two nimesulide preparations.

MATERIALS:

Male Wistar rats (200-250 g) treated with gel containing empty nanocapsules (GEN); gel containing nimesulide in the free form (GNFF) or gel containing nimesulide-loaded nanocapsules (GNN).

METHODS:

Nanoprecipitation methods were used to prepare the colloidal suspension. In-vivo experimental models of paw edema, acute and chronic arthritis, and granuloma formation were used. The gels were applied topically in all models.

RESULTS:

The nanocapsules had an average particle size of 344.6 nm ± 4.33 and a polydispersity index of 0.251 ± 0.002. The models of acute inflammation (paw edema and arthritis) showed that GNFF and GNN were able to inhibit the inflammatory process with similar efficacy. Moreover, nanoencapsulation significantly increased the anti-inflammatory activity of nimesulide in two chronic inflammation models (chronic arthritis and granuloma). The GNFF- and GNN-induced inhibition of inflammation in the chronic arthritis model were 27.75 and 81.5%, respectively. Similarly, GNFF and GNN reduced granuloma formation by 2.82 and 36.66%.

CONCLUSION:

Nanoencapsulation increased the anti-inflammatory activity of topical nimesulide in chronic inflammation models.

• Authors: Quéli Fernandes Lenz, Sílvia Schaffazick Guterres, Adriana Pohlmann, Marta Palma Alves
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 61 Issue 4 Pg. 305-10 (Apr 2012) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 22179794 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Gels
• Nanocapsules
• Sulfonamides
• Carrageenan
• nimesulide

Topics

• Administration, Topical
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage)
• Arthritis, Experimental (drug therapy, pathology)
• Carrageenan
• Edema (chemically induced, drug therapy, pathology)
• Gels
• Granuloma (drug therapy, pathology)
• Male
• Nanocapsules (administration & dosage)
• Rats
• Rats, Wistar
• Sulfonamides (administration & dosage)