Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance.

Abstract

The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.

• Authors: I M F Gjelstad, F Haugen, H L Gulseth, F Norheim, A Jans, S S Bakke, T Raastad, A E Tjønna, U Wisløff, E E Blaak, U Risérus, M Gaster, H M Roche, K I Birkeland, C A Drevon
• Journal: Archives of physiology and biochemistry (Arch Physiol Biochem) Vol. 118 Issue 1 Pg. 22-30 (Feb 2012) ISSN: 1744-4160 [Electronic] England
• PMID: 22117101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Dietary Fats
• Fatty Acids
• PLIN1 protein, human
• Perilipin-1
• Phosphoproteins
• Protein Isoforms
• RNA, Messenger

Topics

• Adipose Tissue
• Aged
• Carrier Proteins (genetics, metabolism)
• Cell Culture Techniques
• Diet
• Dietary Fats (metabolism)
• Energy Metabolism (physiology)
• Exercise (physiology)
• Fatty Acids (pharmacology)
• Female
• Gene Expression (drug effects)
• Humans
• Insulin Resistance
• Male
• Middle Aged
• Muscle Fibers, Skeletal (cytology, drug effects, metabolism)
• Organ Specificity
• Perilipin-1
• Phosphoproteins (genetics, metabolism)
• Physical Endurance (physiology)
• Protein Isoforms (genetics, metabolism)
• RNA, Messenger (biosynthesis)
• Real-Time Polymerase Chain Reaction