[Penehyclidine hydrochloride inhibits glutamate release and related research in global brain ischemia/reperfusion rats].

Abstract

OBJECTIVE:

To investigate the effect of penehyclidine hydrochloride on glutamate (Glu)release and N-methyl-D-aspartate receptor (NMDAR)1 expression in hippocampus CA1 with global cerebral ischemia/reperfusion rats.

METHODS:

Sixty male Wistar rats were randomly allocated into three groups; group A received sham operation; group B received ischemia/reperfusion; group C received penehyclidine hydrochloride treatment (2 mg/kg) before ischemia/reperfusion (n=20). Global cerebral ischemia was induced according to Pulsinelli-Brierley method. All animals were divided into two experiments: (I) Microdialysis plus HPLC/FD were used to detect Glu level after reperfusion 1 h, 3 h, 6 h. (II) After reperfusion 3 h, the animals were decapitated on ice and the brains were immediately removed to detect NMDAR1 expression in CA1 area by immunohistochemistry.

RESULTS:

After penehyclidine hydrochloride treatment, extracellular Glu level in CA1 were significantly decreased compared with those of control group (P < 0.05 or 0.01); Total integrated OD, average gray value and positive-cell area of NMDAR1 in CA1 were also significantly decreased compared with those of control group (P < 0.05 or 0.01).

CONCLUSION:

Penehyclidine hydrochloride might has protective effect in hippocampus CA1 on global cerebral ischemia/reperfusion animals. The protective mechanism might be involved in inhibiting Glu release and NMDAR1 expression.

• Authors: You Shang, Pei-fei Gu, Yu Shang, Yue Li
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 27 Issue 3 Pg. 353-6 (Aug 2011) ISSN: 1000-6834 [Print] China
• PMID: 22097734 (Publication Type: Journal Article)

Chemical References

• Cholinergic Antagonists
• NMDA receptor A1
• Quinuclidines
• Receptors, N-Methyl-D-Aspartate
• penehyclidine
• Glutamic Acid

Topics

• Animals
• Brain Ischemia (metabolism, physiopathology)
• CA1 Region, Hippocampal (metabolism)
• Cholinergic Antagonists (pharmacology)
• Glutamic Acid (metabolism)
• Male
• Quinuclidines (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, N-Methyl-D-Aspartate (metabolism)
• Reperfusion Injury (metabolism, prevention & control)