Abstract | OBJECTIVE: METHODS: (NZB×NZW)F1 mice with SLE were administered human AD-MSCs (5×10(5)) intravenously every 2 weeks from age 6 weeks until age 60 weeks, while the control group received saline vehicle on the same schedule. Another experiment was carried out with a different initiation time point for serial transplantation (age 6 weeks or age 32 weeks). RESULTS: Long-term serial administration (total of 28 times) of human AD-MSCs ameliorated SLE without any adverse effects. Compared with the control group, the human AD-MSC-treated group had a significantly higher survival rate with improvement of histologic and serologic abnormalities and immunologic function, and also had a decreased incidence of proteinuria. Anti- double-stranded DNA antibodies and blood urea nitrogen levels decreased significantly with transplantation of human AD-MSCs, and serum levels of granulocyte-macrophage colony-stimulating factor, interleukin-4 (IL-4), and IL-10 increased significantly. A significant increase in the proportion of CD4+FoxP3+ cells and a marked restoration of capacity for cytokine production were observed in spleens from the human AD-MSC-treated group. In the second experiment, an early stage treatment group showed better results (higher survival rates and lower incidence of proteinuria) than an advanced stage treatment group. CONCLUSION: Serial human AD-MSC transplantation had beneficial effects in the treatment of SLE, without adverse effects. Transplantation of human AD-MSCs before disease onset was preferable for amelioration of SLE and restoration of immune homeostasis.
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Authors | Eun Wha Choi, Il Seob Shin, So Young Park, Ji Hyun Park, Jong Sung Kim, Eun Ji Yoon, Sung Keun Kang, Jeong Chan Ra, Sung Hwa Hong |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 64
Issue 1
Pg. 243-53
(Jan 2012)
ISSN: 1529-0131 [Electronic] United States |
PMID | 21904997
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 by the American College of Rheumatology. |
Chemical References |
- Antibodies, Antinuclear
- Cytokines
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Topics |
- Adipose Tissue
(cytology)
- Animals
- Antibodies, Antinuclear
(immunology, metabolism)
- Cell Count
- Cytokines
(metabolism)
- Disease Models, Animal
- Disease Progression
- Female
- Humans
- Longevity
- Lupus Erythematosus, Systemic
(immunology, pathology, prevention & control)
- Lupus Nephritis
(immunology, pathology, prevention & control)
- Mesenchymal Stem Cell Transplantation
- Mice
- Organ Size
- Proteinuria
(immunology, pathology, prevention & control)
- Spleen
(metabolism, pathology)
- T-Lymphocytes, Regulatory
(immunology, pathology)
- Time Factors
- Transplantation, Heterologous
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