Abstract |
We have identified the membranotropic regions of proteins C and E of DENV virus by performing an exhaustive study of membrane rupture induced by two C and E-derived peptide libraries on model membranes having different phospholipid compositions as well as its ability to modulate the DEPE L(β)-L(α) and L(α)-H(II) phospholipid phase transitions. Protein C presents one hydrophobic leakage-prone region coincidental with a proposed membrane interacting domain, whereas protein E presents five membrane- rupture zones coincidental with different significant zones of the protein, i.e., the fusion peptide, a proline-rich sequence, a sequence containing a hydrophobic pocket as well as the stem and transmembrane domains of the protein. The identification of these membrane-active segments supports their role in viral membrane fusion, formation of the replication complex and morphogenesis and therefore attractive targets for development of new anti-viral compounds.
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Authors | Henrique Nemésio, Francis Palomares-Jerez, José Villalaín |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1808
Issue 10
Pg. 2390-402
(Oct 2011)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 21777566
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Amino Acid Sequence
- Calorimetry, Differential Scanning
- Cell Membrane
(metabolism)
- Dengue Virus
(metabolism)
- Molecular Sequence Data
- Viral Proteins
(chemistry, metabolism)
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