Notoginsenoside ST-4 inhibits virus penetration of herpes simplex virus in vitro.

Abstract

Further study on steam-treated notoginseng, the roots of Panax notoginseng (Burk.) F.H. Chen (Araliaceae), which is a famous traditional Chinese medicine that is used both in raw and treated forms for a long time, led to the isolation of a new dammarane-type saponin, namely notoginsenoside ST-4. Its structure was elucidated to be 3β,12β,20(S)-tri-hydroxydammar-24-ene-3-O-β-d-xylopyranosyl-(1 → 2)-β-d-glucopyranosyl-(1 → 2)-β-d-glu-copyranoside, based on the detailed analyses of the 1D and 2D NMR spectral data and acidic hydrolysis. Notoginsenoside ST-4 was investigated for its antiviral activity on herpes simplex type 1 (HSV-1) and type 2 (HSV-2) in vitro. The 50% effective concentration (EC(50)) values, determined by plaque reduction assay, were 16.47 ± 0.67 and 19.44 ± 1.16 μM for HSV-1 and HSV-2, respectively, whereas the 50% cytotoxic concentration (CC(50)) determined by the XTT test on Vero cells was 510.64 ± 4.56 μM. As analyzed by attachment assay and penetration assay based on plaque reduction assay, the antiviral activity of notoginsenoside ST-4 was principally due to the penetration inhibition effects, which was confirmed by fluorescence microscopy observation that notoginsenoside ST-4 blocked the penetration of virus. Therefore, notoginsenoside ST-4 might be a promising agent for herpes simplex virus infection.

• Authors: Ying Pei, Qian Du, Peng-Ying Liao, Zhen-Ping Chen, Dong Wang, Chong-Ren Yang, Kaio Kitazato, Yi-Fei Wang, Ying-Jun Zhang
• Journal: Journal of Asian natural products research (J Asian Nat Prod Res) Vol. 13 Issue 6 Pg. 498-504 (Jun 2011) ISSN: 1477-2213 [Electronic] England
• PMID: 21623512 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Drugs, Chinese Herbal
• Saponins
• Triterpenes
• notoginsenoside ST-4

Topics

• Animals
• Antiviral Agents (chemistry, isolation & purification, pharmacology)
• Chlorocebus aethiops
• Drugs, Chinese Herbal (chemistry, isolation & purification, pharmacology)
• Molecular Structure
• Panax (chemistry)
• Plant Roots (chemistry)
• Saponins (chemistry, isolation & purification, pharmacology)
• Simplexvirus (drug effects)
• Triterpenes (chemistry, isolation & purification, pharmacology)
• Vero Cells