Associations of common polymorphisms in GCKR with type 2 diabetes and related traits in a Han Chinese population: a case-control study.

Abstract

BACKGROUND:

Several studies have shown that variants in the glucokinase regulatory protein gene (GCKR) were associated with type 2 diabetes and dyslipidemia. The purpose of this study was to examine whether tag single nucleotide polymorphisms (SNPs) in the GCKR region were associated with type 2 diabetes and related traits in a Han Chinese population and to identify the potential mechanisms underlying these associations.

METHODS:

We investigated the association of polymorphisms in the GCKR gene with type 2 diabetes by employing a case-control study design (1118 cases and 1161 controls). Four tag SNPs (rs8179206, rs2293572, rs3817588 and rs780094) with pairwise r2>0.8 and minor allele frequency>0.05 across the GCKR gene and its flanking regions were studied and haplotypes were constructed. Genotyping was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy using a MassARRAY platform.

RESULTS:

The G alleles of GCKR rs3817588 and rs780094 were associated with an increased risk of type 2 diabetes after adjustment for year of birth, sex and BMI (OR=1.24, 95% CI 1.08-1.43, p=0.002 and OR=1.22, 95% CI 1.07-1.38, p=0.002, respectively). In the non-diabetic controls, the GG carriers of rs3817588 and rs780094 were nominally associated with a lower plasma triglyceride level compared to the AA carriers after adjustment for year of birth, sex and BMI (p for trend=0.00004 and 0.03, respectively). Furthermore, the association of rs3817588 with plasma triglyceride level was still significant after correcting for multiple testing.

CONCLUSIONS:

The rs3817588 A/G polymorphism of the GCKR gene was associated with type 2 diabetes and plasma triglyceride level in the Han Chinese population.

• Authors: Yan Ling, Xiaomu Li, Qian Gu, Hongyan Chen, Daru Lu, Xin Gao
• Journal: BMC medical genetics (BMC Med Genet) Vol. 12 Pg. 66 (May 13 2011) ISSN: 1471-2350 [Electronic] England
• PMID: 21569451 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• Blood Glucose
• Cholesterol, HDL
• Cholesterol, LDL
• GCKR protein, human
• Triglycerides
• Cholesterol

Topics

• Adaptor Proteins, Signal Transducing (genetics)
• Asian People (genetics)
• Blood Glucose (genetics)
• Blood Pressure (genetics)
• Case-Control Studies
• China
• Cholesterol (blood, genetics)
• Cholesterol, HDL (blood, genetics)
• Cholesterol, LDL (blood, genetics)
• Diabetes Mellitus, Type 2 (genetics)
• Female
• Gene Frequency
• Genotype
• Haplotypes (genetics)
• Humans
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Triglycerides (blood, genetics)
• Waist Circumference