Abstract | INTRODUCTION: METHODS: Polymicrobiological abdominal sepsis was induced by cecal ligation and puncture (CLP). Mice were treated with either 1 μg of intravenous rhAng-1 or control buffer immediately after CLP induction and every 8h thereafter. Sham-operated animals served as time-matched controls. RESULTS: Compared to buffer-treated controls, rhAng-1 treated septic mice showed significant improvements in several hematologic and biochemical indicators of MODS. Moreover, rhAng-1 stabilized endothelial barrier function, as evidenced by inhibition of protein leakage from lung capillaries into the alveolar compartment. Histological analysis revealed that rhAng-1 treatment attenuated leukocyte infiltration in lungs and kidneys of septic mice, probably due to reduced endothelial adhesion molecule expression in rhAng-1 treated mice. Finally, the protective effects of rhAng-1 treatment were reflected by an improved survival time in a lethal CLP model. CONCLUSIONS: In a clinically relevant murine sepsis model, intravenous rhAng-1 treatment alone is sufficient to significantly improve a variety of sepsis-associated organ dysfunctions and survival time, most likely by preserving endothelial barrier function. Further studies are needed to pave the road for clinical application of this therapy concept.
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Authors | Sascha David, Joon-Keun Park, Matijs van Meurs, Jan G Zijlstra, Christian Koenecke, Claudia Schrimpf, Nelli Shushakova, Faikah Gueler, Hermann Haller, Philipp Kümpers |
Journal | Cytokine
(Cytokine)
Vol. 55
Issue 2
Pg. 251-9
(Aug 2011)
ISSN: 1096-0023 [Electronic] England |
PMID | 21531574
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- Angiopoietin-1
- Angiopoietin-2
- Recombinant Proteins
- Receptor Protein-Tyrosine Kinases
- Receptor, TIE-2
- Tek protein, mouse
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Topics |
- Angiopoietin-1
(genetics, metabolism, therapeutic use)
- Angiopoietin-2
(metabolism)
- Animals
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Humans
- Mice
- Multiple Organ Failure
(drug therapy, etiology, pathology)
- Receptor Protein-Tyrosine Kinases
(metabolism)
- Receptor, TIE-2
- Recombinant Proteins
(genetics, therapeutic use)
- Sepsis
(complications, drug therapy, mortality, pathology)
- Survival Rate
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