The localisation of PtdIns3P in Drosophila fat responds to nutrients but not insulin: a role for Class III but not Class II phosphoinositide 3-kinases.

Abstract	PtdIns3P and PtdIns(3,4,5)P(3) are regulated differently in fat body in response to nutritional status and insulin signalling. In feeding larvae PtdIns(3,4,5)P(3) is upregulated at the cell membrane where it is generated in response to insulin signalling. In contrast PtdIns3P is down regulated in the fat body of well-fed larvae but on starvation it accumulates in punctate vesicles throughout the cytoplasm and on refeeding it relocalises to vesicles at the periphery of the cell. Both responses are independent of insulin signalling and on the presence of glutamine which has previously been linked to nutritional sensing. We find that both Class II and Class III PI3Ks are capable of generating PtdIns3P in vivo but the source of PtdIns3P in the fat body and the response to nutritional status can be exclusively accounted for by Class III PI3K activity.
Authors	Katie V Powis, Lindsay K MacDougall
Journal	Cellular signalling (Cell Signal) Vol. 23 Issue 7 Pg. 1153-61 (Jul 2011) ISSN: 1873-3913 [Electronic] England
PMID	21385607 (Publication Type: Journal Article)
Copyright	Copyright © 2011 Elsevier Inc. All rights reserved.
Chemical References	Insulin Phosphatidylinositol Phosphates Recombinant Proteins phosphatidylinositol 3-phosphate Glutamine Green Fluorescent Proteins Class II Phosphatidylinositol 3-Kinases Class III Phosphatidylinositol 3-Kinases Receptor, Insulin
Topics	Adipocytes (drug effects, metabolism) Animal Nutritional Physiological Phenomena Animals Cell Membrane (drug effects, metabolism) Class II Phosphatidylinositol 3-Kinases (metabolism) Class III Phosphatidylinositol 3-Kinases (metabolism) Drosophila melanogaster (enzymology, metabolism) Fat Body (metabolism) Food Gene Expression Regulation, Developmental Genes, Reporter Glutamine (pharmacology) Green Fluorescent Proteins (biosynthesis) Insulin (pharmacology) Larva (enzymology, metabolism) Phosphatidylinositol Phosphates (metabolism) Receptor, Insulin (biosynthesis) Recombinant Proteins (biosynthesis) Signal Transduction Starvation (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!