Towards a mouse model for sickle cell disease: HB SAD.

Abstract

Very recently a high expression of human hemoglobin S, which causes sickle cell disease, has been obtained in transgenic mice. We have constructed a modified beta S gene, beta SAD which carries two additional mutations in order to induce polymerization of transgenic hemoglobin when diluted by endogenous mouse Hb. The transgenic SAD mice are not anemic but exhibit a low percentage of irreversible sickle cells. Sickling is induced by deoxygenation of erythrocytes in vitro. In addition, the anemia of neonates and the low incidence of SAD animals in the progeny suggest a deleterious effect of SAD Hb during development. Finally, hypoxia induces a high mortality in SAD adults suggesting the induction of vaso-occlusive events.

• Authors: M Trudel, M C Garel, N Saadane, P Rouyer-Fessard, D Vidaud, F Costantini, Y Beuzard
• Journal: Nouvelle revue francaise d'hematologie (Nouv Rev Fr Hematol (1978)) Vol. 32 Issue 6 Pg. 407-8 ( 1990) Germany
• PMID: 2101873 (Publication Type: Journal Article)

Chemical References

• Hemoglobin, Sickle
• Recombinant Proteins
• hemoglobin SAD
• Globins

Topics

• Anemia, Sickle Cell
• Animals
• Animals, Newborn (blood)
• Disease Models, Animal
• Globins (genetics)
• Hemoglobin, Sickle (genetics)
• Hypoxia (complications)
• Mice
• Mice, Transgenic (blood, genetics)
• Protein Engineering
• Recombinant Proteins
• Thromboembolism (etiology)