Association of mouse Dlg4 (PSD-95) gene deletion and human DLG4 gene variation with phenotypes relevant to autism spectrum disorders and Williams' syndrome.

Abstract

OBJECTIVE:

Research is increasingly linking autism spectrum disorders and other neurodevelopmental disorders to synaptic abnormalities ("synaptopathies"). PSD-95 (postsynaptic density-95, DLG4) orchestrates protein-protein interactions at excitatory synapses and is a major functional bridge interconnecting a neurexinneuroligin-SHANK pathway implicated in autism spectrum disorders.

METHOD:

The authors characterized behavioral, dendritic, and molecular phenotypic abnormalities relevant to autism spectrum disorders in mice with PSD-95 deletion (Dlg4⁻(/)⁻). The data from mice led to the identification of single-nucleotide polymorphisms (SNPs) in human DLG4 and the examination of associations between these variants and neural signatures of Williams' syndrome in a normal population, using functional and structural neuroimaging.

RESULTS:

Dlg4⁻(/)⁻ showed increased repetitive behaviors, abnormal communication and social behaviors, impaired motor coordination, and increased stress reactivity and anxiety-related responses. Dlg4⁻(/)⁻ had subtle dysmorphology of amygdala dendritic spines and altered forebrain expression of various synaptic genes, including Cyln2, which regulates cytoskeletal dynamics and is a candidate gene for Williams' syndrome. A signifi-cant association was observed between variations in two human DLG4 SNPs and reduced intraparietal sulcus volume and abnormal cortico-amygdala coupling, both of which characterize Williams' syndrome.

CONCLUSIONS:

These findings demonstrate that DLG4 gene disruption in mice produces a complex range of behavioral and molecular abnormalities relevant to autism spectrum disorders and Williams' syndrome. The study provides an initial link between human DLG4 gene variation and key neural endophenotypes of Williams' syndrome and perhaps corticoamygdala regulation of emotional and social processes more generally.

• Authors: Michael Feyder, Rose-Marie Karlsson, Poonam Mathur, Matthew Lyman, Roland Bock, Reza Momenan, Jeeva Munasinghe, Maria Luisa Scattoni, Jessica Ihne, Marguerite Camp, Carolyn Graybeal, Douglas Strathdee, Alison Begg, Veronica A Alvarez, Peter Kirsch, Marcella Rietschel, Sven Cichon, Henrik Walter, Andreas Meyer-Lindenberg, Seth G N Grant, Andrew Holmes
• Journal: The American journal of psychiatry (Am J Psychiatry) Vol. 167 Issue 12 Pg. 1508-17 (Dec 2010) ISSN: 1535-7228 [Electronic] United States
• PMID: 20952458 (Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)

Chemical References

• DLG4 protein, human
• Disks Large Homolog 4 Protein
• Dlg4 protein, mouse
• Intracellular Signaling Peptides and Proteins
• Membrane Proteins
• Nerve Tissue Proteins
• Guanylate Kinases

Topics

• Adult
• Amygdala (pathology, physiopathology, ultrastructure)
• Animals
• Behavior, Animal
• Child
• Child Development Disorders, Pervasive (genetics, pathology)
• Dendritic Spines (ultrastructure)
• Disease Models, Animal
• Disks Large Homolog 4 Protein
• Female
• Gene Deletion
• Genetic Variation
• Guanylate Kinases
• Humans
• Intracellular Signaling Peptides and Proteins (genetics)
• Male
• Membrane Proteins (genetics)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Nerve Tissue Proteins (metabolism)
• Neural Pathways (pathology)
• Parietal Lobe (pathology)
• Phenotype
• Polymorphism, Single Nucleotide
• Prosencephalon (metabolism)
• Williams Syndrome (genetics, pathology, physiopathology)