Effect of Piper betle on cardiac function, marker enzymes, and oxidative stress in isoproterenol-induced cardiotoxicity in rats.

Abstract

The present study was designed to investigate the cardioprotective potential of Piper betle (P. betle) against isoproterenol (ISP)-induced myocardial infarction in rats. Rats were randomly divided into eight groups viz. control, ISP, P. betle (75, 150, and 300 mg/kg) and P. betle (75, 150, and 300 mg/kg) + ISP treated group. P. betle leaf extract (75, 150, or 300 mg/kg) or saline was orally administered for 30 days. ISP (85 mg/kg, s.c.) was administered at an interval of 24 h on the 28(th) and 29(th) day and on day 30 the functional and biochemical parameters were measured. ISP administration showed a significant decrease in systolic, diastolic, mean arterial pressure (SAP, DAP, MAP), heart rate (HR), contractility (+LVdP/dt), and relaxation (-LVdP/dt) and increased left ventricular end-diastolic pressure (LVEDP). ISP also caused significant decrease in myocardial antioxidants; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and myocyte injury marker enzymes; creatine phosphokinase-MB (CK-MB) isoenzyme and lactate dehydrogenase (LDH) along with enhanced lipid peroxidation; thiobarbituric acid reacting species (TBARS) in heart. Pre-treatment with P. betle favorably modulated hemodynamic (SAP, DAP, and MAP) and ventricular function parameters (-LVdP/dt and LVEDP). P. betle pre-treatment also restored SOD, CAT, GSH, and GPx, reduced the leakage of CK-MB isoenzyme and LDH along with decreased lipid peroxidation in the heart. Taken together, the biochemical and functional parameters indicate that P. betle 150 and 300 mg/kg has a significant cardioprotective effect against ISP-induced myocardial infarction. Results of the present study suggest the cardioprotective potential of P. betle.

• Authors: Dharamvir Singh Arya, Sachin Arora, Salma Malik, Saroj Nepal, Santosh Kumari, Shreesh Ojha
• Journal: Toxicology mechanisms and methods (Toxicol Mech Methods) Vol. 20 Issue 9 Pg. 564-71 (Nov 2010) ISSN: 1537-6524 [Electronic] England
• PMID: 20846025 (Publication Type: Journal Article)

Chemical References

• Cardiotonic Agents
• Cardiotoxins
• Plant Extracts
• Thiobarbituric Acid Reactive Substances
• Glutathione
• Isoproterenol

Topics

• Animals
• Blood Pressure (drug effects)
• Cardiotonic Agents (pharmacology)
• Cardiotoxins (toxicity)
• Glutathione (metabolism)
• Heart (drug effects)
• Isoproterenol (toxicity)
• Male
• Myocardium (enzymology, metabolism)
• Oxidative Stress (drug effects)
• Piper betle
• Plant Extracts (pharmacology)
• Rats
• Rats, Wistar
• Thiobarbituric Acid Reactive Substances (metabolism)