Abstract |
An excessive activation of the excitatory amino acid system has been proposed as one possible mediator of the ischemia-induced delayed death of CA1 pyramidal cells in the hippocampus. Using dialytrodes in the CA1 of the rat, we have investigated multiple-unit activity and extracellular changes in acidic sulfur-containing amino acids and gamma-glutamyl peptides during ischemia (20-min, four-vessel occlusion) and during 8 h of reflow. Multiple-unit activity was abolished during ischemia and for the following 1 h, but then recovered, gradually reaching preischemic levels after 8 h of reflow. Extracellular cysteate, cysteine sulfinate, and gamma-glutamyltaurine increased (1.5- to threefold) during ischemia, and extracellular glutathione and gamma- glutamylaspartate plus gamma-glutamylglutamine increased during early reflow (two- to threefold). The recovery of neuronal activity at 4-8 h was paralleled by an increase in extracellular cysteine sulfinate (2.5-fold at 8 h of reflow). Perfusion with 10 microM tetrodotoxin at 8 h of reflow abolished the multiple-unit activity and reduced extracellular cysteine sulfinate. Considering the glutamate-like properties of cysteine sulfinate, the observed postischemic increase may be involved in the development of the delayed neuronal death.
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Authors | P Andiné, O Orwar, I Jacobson, M Sandberg, H Hagberg |
Journal | Journal of neurochemistry
(J Neurochem)
Vol. 57
Issue 1
Pg. 230-6
(Jul 1991)
ISSN: 0022-3042 [Print] England |
PMID | 2051166
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acids
- Amino Acids
- Dipeptides
- Neurotransmitter Agents
- Glutamine
- gamma-glutamylglutamine
- gamma-glutamylaspartic acid
- Taurine
- Tetrodotoxin
- Sulfur
- glutaurine
- Cysteine
- cysteine sulfinic acid
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Topics |
- Acids
(metabolism)
- Amino Acids
(metabolism)
- Animals
- Brain Ischemia
(metabolism)
- Cysteine
(analogs & derivatives, metabolism)
- Dipeptides
(metabolism)
- Electrochemistry
- Extracellular Space
(metabolism)
- Glutamine
(analogs & derivatives, metabolism)
- Hippocampus
(cytology, metabolism)
- Male
- Neurons
(physiology)
- Neurotransmitter Agents
- Rats
- Rats, Inbred Strains
- Sulfur
(metabolism)
- Taurine
(analogs & derivatives, metabolism)
- Tetrodotoxin
(pharmacology)
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