[Neuroprotective mechanism of tetrahydroxystilbene glucoside on rats after cerebral ischemia-reperfusion].

Abstract

OBJECTIVE:

To investigate the neuroprotective mechanism of tetrahydroxystilbene glucoside (TSG), a Chinese medicine, on rats after cerebral ischemia-reperfusion.

METHODS:

A total of 96 Sprague-Dawley male rats were divided into 4 groups (n=24): a control group, an ischemia-reperfusion (I/R) model group, a low dose TSG [60 mg/(kg.d)]group, and a high dose TSG [120 mg/(kg.d)]group. After 6 days intragastric (ig) administration of TSG or natural saline (I/R group), reversible middle cerebral artery occlusion (MCAO) model was established by intraluminal suture technique. The rats of control group were operated on while the middle cerebral artery was not blocked. At 6 h, 24 h, 48 h, and 7 d after the reperfusion, behavior test was used to evaluate the neurological deficiency of each group. The protein expressions of nerve growth factor (NGF), growth associated protein (GAP)-43, and protein kinase A catalytic subunit (PKAc) in the cortex were measured by immunohistochemical method.

RESULTS:

Compared with the I/R group, the neurological defect scores of the 2 TSG groups were significantly lower except at 6 h after the reperfusion. Compared with the I/R group, the protein expression of NGF, GAP-43, and PKAc after the reperfusion of the 2 TSG groups increased significantly.

CONCLUSION:

The protein expression of NGF may increase when treated with TSG after cerebral ischemia-reperfusion, which activates the PKA pathway and increases the protein expression of GAP-43 that protects the neuron.

• Authors: Jie Yang, Zhiwen Zhou, Qidong Yang, Lijun Zheng, Jin Zeng
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 35 Issue 4 Pg. 321-8 (Apr 2010) ISSN: 1672-7347 [Print] China
• PMID: 20448353 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GAP-43 Protein
• Glucosides
• Neuroprotective Agents
• Stilbenes
• 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside
• Nerve Growth Factor

Topics

• Animals
• GAP-43 Protein (metabolism)
• Glucosides (pharmacology, therapeutic use)
• Infarction, Middle Cerebral Artery (complications, drug therapy)
• Male
• Nerve Growth Factor (metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)
• Stilbenes (pharmacology, therapeutic use)