Abstract | UNLABELLED: AIM: Assess the expression of cytokines IL not4, IL not5, IL not6, IL not8, GM not-CSF and IFN not-y, analyzed through RT-PCR, in the polyps of patients with cystic fibrosis. MATERIALS AND METHODS: A cross-sectional, prospective study was carried out with 24 patients, 13 of whom had cystic fibrosis and nasal polyposis ( Cystic Fibrosis Group) and 11 had normal otorhinolaryngological exams (Control Group). The average age was 21 years (3 not57); 12 participants were males and 12 were females. The cytokine profile was studied in mucosal fragments (Control Group) or nasal polyps ( Cystic Fibrosis Group) through RT notPCR. Transcriptions were studied for cytokines IL not4, IL not5, IL not6, IL not8, IFN noty and GM notCSF, adjusted for the beta not-actin value. RESULTS:
Interleukins 5, 6, 8 and GM notCSF were similar in both groups (p>0.05). There were lower values of IFN-y not (p=0.03) and a strong tendency toward an increase in IL not4 (p=0.06) in the Cystic Fibrosis Group. CONCLUSION: Inflammatory and structural cells may produce messenger RNA for IL not4, blocking the production of other cytokines such as IFN-y, suggesting the participation of this mechanism in the formation of polyps in cystic fibrosis.
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Authors | Flávio Barbosa Nunes, Mirian Cabral Moreira de Castro, Tacimara Moreira da Silva, Ricardo Nascimento Araújo, Helena Maria Gonçalves Becker, Paulo Fernando Tormin Borges Crosara, Roberto Eustáquio Santos Guimarães |
Journal | Brazilian journal of otorhinolaryngology
(Braz J Otorhinolaryngol)
2010 Jan-Feb
Vol. 76
Issue 1
Pg. 25-8
ISSN: 1808-8686 [Electronic] Brazil |
PMID | 20339685
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Biomarkers
- Cytokines
- RNA, Messenger
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Topics |
- Adolescent
- Biomarkers
(blood)
- Case-Control Studies
- Child
- Cross-Sectional Studies
- Cystic Fibrosis
(complications, immunology)
- Cytokines
(blood)
- Female
- Humans
- Male
- Middle Aged
- Nasal Polyps
(complications, immunology)
- Prospective Studies
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Young Adult
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