Metabolomic analysis of serum by (1) H NMR spectroscopy in amyotrophic lateral sclerosis.

Abstract

BACKGROUND:

Amyotrophic lateral sclerosis (ALS), an invariably fatal neurological disorder shows complicated pathogenesis that poses challenges with respect to diagnosis as well as monitoring of disease progression.

METHODS:

We investigated metabolite profiles in the serum of 30 patients with ALS, 10 patients of Hirayama disease, which served as a neurological disease control and 25 healthy controls by using (1) H NMR spectroscopy.

RESULTS:

Compared to healthy controls, the ALS patients had higher quantities of glutamate (P<0.001), beta-hydroxybutyrate (P<0.001), acetate (P<0.01), acetone (P<0.05), and formate (P<0.001), and lower concentrations of glutamine (P<0.02), histidine (P<0.001) and N-acetyl derivatives. On the other hand, Hirayama disease patients had significantly higher median concentrations of pyruvate (P<0.05), glutamate (P<0.001), formate (P<0.05) and lower median concentrations of N-acetyl derivatives. Furthermore, we also found that serum glutamate showed a positive correlation (P<0.001, r=0.6487) whereas, histidine showed a negative correlation (P<0.001, r=-0.5641) with the duration of the disease in ALS.

CONCLUSIONS:

Such (1) H NMR study of serum may reveal abnormal metabolite patterns, which could have the potential to serve as surrogate markers for monitoring ALS disease progression.

• Authors: Alok Kumar, Lakshmi Bala, Jayantee Kalita, U K Misra, R L Singh, C L Khetrapal, G Nagesh Babu
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 411 Issue 7-8 Pg. 563-7 (Apr 02 2010) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 20096678 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2009 Elsevier B.V. All rights reserved.

Chemical References

• Acetates
• Formates
• Protons
• Glutamine
• Acetone
• Glutamic Acid
• Histidine
• 3-Hydroxybutyric Acid

Topics

• 3-Hydroxybutyric Acid (blood)
• Acetates
• Acetone (blood)
• Adult
• Amyotrophic Lateral Sclerosis (blood)
• Female
• Formates (blood)
• Glutamic Acid (blood)
• Glutamine (blood)
• Histidine (blood)
• Humans
• Magnetic Resonance Spectroscopy
• Male
• Protons
• Sensitivity and Specificity