Identification of a novel splicing isoform of murine CGI-58.

Abstract

The comparative gene identification-58 (CGI-58) gene, mutations of which are linked to Chanarin-Dorfman syndrome, encodes a protein of the alpha/beta hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI-58 gene, termed mCGI-58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full-length protein displayed perilipin-dependent localization to lipid droplets, mCGI-58S showed a predominant cytoplasmic staining when expressed in cells. mCGI-58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI-58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.

• Authors: Xingyuan Yang, Xin Lu, Jun Liu
• Journal: FEBS letters (FEBS Lett) Vol. 584 Issue 5 Pg. 903-10 (Mar 05 2010) ISSN: 1873-3468 [Electronic] England
• PMID: 20083112 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• Protein Isoforms
• Triglycerides
• 1-Acylglycerol-3-Phosphate O-Acyltransferase
• Abhd5 protein, mouse
• Carboxylic Ester Hydrolases
• Lipase
• PNPLA2 protein, mouse

Topics

• 1-Acylglycerol-3-Phosphate O-Acyltransferase (genetics, metabolism)
• 3T3-L1 Cells
• Animals
• Carboxylic Ester Hydrolases (metabolism)
• Fluorescent Antibody Technique
• HeLa Cells
• Humans
• Immunoblotting
• Lipase
• Lipid Metabolism (genetics, physiology)
• Mice
• Microscopy, Confocal
• Protein Isoforms (genetics, metabolism)
• RNA Splicing (genetics, physiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Triglycerides (metabolism)