Yellow fever mosquito sterol carrier protein-2 gene structure and transcriptional regulation.

Abstract

AeSCP-2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP-2 gene is important for mosquito development. An earlier study demonstrated that the transcription of this gene was upregulated by 20-hydroxyecdysone (20E) in cultured gut tissues. To investigate 20E-regulated transcription of the AeSCP-2 gene we truncated the upstream flanking region of AeSCP-2 gene and linked it to a reporter gene. The mosquito Aag-2 cell line was transfected with these promoter/reporter constructs and treated with 20E at various concentrations. Expression vectors of different transcription factors such as HR3 and beta FTZ-F1 were also co-transfected with the AeSCP-2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP-2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating the transcriptional activity of the AeSCP-2 gene.

• Authors: I Vyazunova, Q Lan
• Journal: Insect molecular biology (Insect Mol Biol) Vol. 19 Issue 2 Pg. 205-15 (Apr 2010) ISSN: 1365-2583 [Electronic] England
• PMID: 20002221 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• DNA Primers
• HR3 protein, Aedes aegypti
• Insect Proteins
• RNA, Messenger
• Receptors, Cytoplasmic and Nuclear
• Steroidogenic Factor 1
• Transcription Factors
• sterol carrier proteins
• Ecdysterone
• DNA

Topics

• Aedes (drug effects, genetics, metabolism)
• Animals
• Animals, Genetically Modified
• Base Sequence
• Carrier Proteins (genetics)
• Cell Line
• DNA (genetics, metabolism)
• DNA Primers (genetics)
• Ecdysterone (pharmacology)
• Genes, Insect (drug effects)
• Insect Proteins (genetics)
• Promoter Regions, Genetic
• RNA, Messenger (genetics, metabolism)
• Receptors, Cytoplasmic and Nuclear (genetics)
• Steroidogenic Factor 1 (genetics, metabolism)
• Tissue Culture Techniques
• Transcription Factors (metabolism)
• Transcription, Genetic (drug effects)
• Transfection