Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins.

Abstract

An enzyme labeled immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins were developed and a comparative evaluation was performed. A polyclonal antibody (BC67) used in both assay formats was raised to saxitoxin-jeffamine-BSA in New Zealand white rabbits. Each assay format was designed as an inhibition assay. Shellfish samples (n=54) were evaluated by each method using two simple rapid extraction procedures and compared to the AOAC high performance liquid chromatography (HPLC) and the mouse bioassay (MBA). The results of each assay format were comparable with the HPLC and MBA methods and demonstrate that an antibody with high sensitivity and broad specificity to PSP toxins can be applied to different immunological techniques. The method of choice will depend on the end-users needs. The reduced manual labor and simplicity of operation of the SPR biosensor compared to ELISA, ease of sample extraction and superior real time semi-quantitative analysis are key features that could make this technology applicable in a high-throughput monitoring unit.

• Authors: Katrina Campbell, Anne-Catherine Huet, Caroline Charlier, Cowan Higgins, Philippe Delahaut, Christopher T Elliott
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 877 Issue 32 Pg. 4079-89 (Dec 15 2009) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 19926541 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study)

Chemical References

• Saxitoxin

Topics

• Animals
• Enzyme-Linked Immunosorbent Assay
• Limit of Detection
• Mice
• Saxitoxin (analysis, immunology)
• Shellfish (analysis)
• Surface Plasmon Resonance (economics, methods)