Small-molecule suppression of misfolding of mutated human carbonic anhydrase II linked to marble brain disease.

Abstract

Carbonic anhydrase II deficiency syndrome or Marble brain disease (MBD) is caused by autosomal recessive mutations in the human carbonic anhydrase II (HCA II) gene. Here we report a small-molecule stabilization study of the exceptionally destabilized HCA II mutant H107Y employing inhibitors based on p-aminobenzoylsulfonamide compounds and 1,3,4-thiadiazolylsulfonamides as well as amino acid activators. Protein stability assays showed a significant stabilization by the aromatic sulfonamide inhibitors when present at 10 microM concentration, providing shifts of the midpoint of thermal denaturation between 10 degrees C and 16 degrees C and increasing the free energies of denaturation 0.5-3.0 kcal/mol as deduced from GuHCl denaturation. This study could be used as a starting point for the design of small-molecule folding modulators and possibly autoactivatable molecules for suppression of misfolding of destabilized HCA II mutants.

• Authors: Karin Almstedt, Therese Rafstedt, Claudiu T Supuran, Uno Carlsson, Per Hammarström
• Journal: Biochemistry (Biochemistry) Vol. 48 Issue 23 Pg. 5358-64 (Jun 16 2009) ISSN: 1520-4995 [Electronic] United States
• PMID: 19415900 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carbonic Anhydrase Inhibitors
• Sulfonamides
• Carbonic Anhydrase II

Topics

• Brain Diseases, Metabolic, Inborn (genetics)
• Carbonic Anhydrase II (antagonists & inhibitors, chemistry, genetics)
• Carbonic Anhydrase Inhibitors (pharmacology)
• Humans
• Kinetics
• Models, Molecular
• Protein Conformation
• Protein Denaturation
• Protein Folding
• Sulfonamides (chemistry, pharmacology)
• Thermodynamics