Abstract | OBJECTIVE: METHODS: Sixty-four male Kunming mice were randomly divided into normal control (A) group, exposed (B) group, recombinant adenoviruses 5 of IGF-1 (Ad5-IGF-1) intervention (C) group (in which Ad5-IGF-1 was injected into the trachea of the mice), blank vector control (D) group. B, C and D groups were exposed to gaseous PFIB in a flow-past whole-body exposure system. The lung index, concentration of total protein and albumin in bronchoalveolar lavage fluid (BALF), concentration of IGF-1 in serum and lung homogenate were measured. The lung pathologic changes were examined with light microscope, and ultrastructure changes in alveolar type II cells (ATII) with electron microscope. RESULTS: Compared with A group, the lung index, concentration of total protein in BALF were significantly increased in other groups, the lung index and concentration of total protein and albumin of BALF in B and D groups were prominently higher than C group (all P<0.01). The concentration of IGF-1 in serum of B and D groups was lower markedly than that of A group, and the concentration of IGF-1 in serum of C group was distinctly higher than those of A, B, D groups (all P<0.01). The concentration of IGF-1 in lung homogenate of B, C, D groups was higher than that of A group, and the concentration of IGF-1 in lung homogenate of C group was significantly higher than that of B and D groups (all P<0.01). Lung hyaline membrane formation, diffuse alveolar atelectasis, accumulation of edema fluid, red blood cell exudation, were obviously milder in C group, and changes in the ultrastructure of ATII showed a similar result. CONCLUSION: The protective effect of Ad5-IGF-1 against the toxicity of PFIB inhalation is identified. In the mice pretreated with Ad5-IGF-1 is able to significantly lower lung index, the protein concentration in BALF, and the concentration of IGF-1 in serum and lung homogenate is obviously increased. Protection of ATII may be one of the mechanisms.
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Authors | Bei Li, Li Chen, Jin-fu Ma, Jian Zhao, Ming Yin, Ri-gao Ding, Tan-shi Li |
Journal | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
(Zhongguo Wei Zhong Bing Ji Jiu Yi Xue)
Vol. 21
Issue 4
Pg. 219-21
(Apr 2009)
ISSN: 1003-0603 [Print] China |
PMID | 19374789
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorocarbons
- perfluoroisobutylene
- Insulin-Like Growth Factor I
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Topics |
- Acute Lung Injury
(chemically induced, prevention & control)
- Adenoviridae
(genetics)
- Animals
- Bronchoalveolar Lavage Fluid
(chemistry)
- Disease Models, Animal
- Epithelial Cells
(pathology)
- Fluorocarbons
(toxicity)
- Genetic Vectors
- Insulin-Like Growth Factor I
(genetics, metabolism)
- Lung
(metabolism, pathology)
- Male
- Mice
- Pulmonary Alveoli
(pathology)
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