Abstract | BACKGROUND: METHODS: Muscle strips (n=90) were isolated from human right atria (n=46). Contractures were induced by oxygen and glucose withdrawal. In 18 muscle strips contractures were prevented by means of butanedione monoxime (BDM). Sarcomere lengths were measured by electron microscopy (n=12). The gene expression and protein amount of BNP were determined and compared to control muscle strips contracting under physiological conditions. RESULTS:
Hypoxia significantly decreased systolic force and induced diastolic contractures. This mechanical stress could be prevented in the group treated with BDM as evidenced by electron microscopy. Ischemia significantly increased BNP expression in both groups as evidenced by Northern blot analysis and immunohistochemistry. This increase was independent from mechanical stress. CONCLUSION: Our results indicate that ischemia is a potent mechanism for the expression of BNP. The increase in BNP expression under ischemic conditions is independent from concomitant mechanical alterations.
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Authors | Helge Möllmann, Holger M Nef, Sawa Kostin, Adrian Dragu, Christoph Maack, Michael Weber, Christian Troidl, Andreas Rolf, Albrecht Elsässer, Michael Böhm, Regina Brantner, Christian W Hamm, Christian J F Holubarsch |
Journal | International journal of cardiology
(Int J Cardiol)
Vol. 143
Issue 3
Pg. 289-97
(Sep 03 2010)
ISSN: 1874-1754 [Electronic] Netherlands |
PMID | 19329198
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2009 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Natriuretic Peptide, Brain
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Topics |
- Aged
- Atrial Appendage
(metabolism, ultrastructure)
- Diastole
(physiology)
- Female
- Heart Failure
(metabolism, physiopathology)
- Humans
- Hypoxia
(metabolism, physiopathology)
- Male
- Microscopy, Electron
- Middle Aged
- Myocardial Ischemia
(metabolism, physiopathology)
- Myocardium
(metabolism, ultrastructure)
- Natriuretic Peptide, Brain
(metabolism)
- Stress, Mechanical
- Systole
(physiology)
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