Cyclosporin A blocks surface IgM-mediated growth inhibition in an immature B lymphoma, BKS-2.

Abstract

Cyclosporin A (CSA) is an immunosuppressive drug, which blocks selective activation pathways in T and B cells. Antigen receptor-mediated signaling events have been shown to be very sensitive to CSA. Signaling through the surface IgM receptor had been shown to induce growth inhibition in immature B cell lymphoma cells. In this report, we demonstrate that CSA caused significant reversal of growth inhibition induced by an anti-mu antibody in an immature B lymphoma, BKS-2. Time-course experiments indicated that CSA was completely effective when added as late as 4 h after the addition of ligand. CSA was also found to have no direct effect on anti-mu-induced Ca2+ elevation. These results suggest that the likely target for CSA lies downstream from the initial generation of second messengers, such as Ca2+.

• Authors: V Udhayakumar, S Muthukkumar, B Subbarao
• Journal: European journal of immunology (Eur J Immunol) Vol. 21 Issue 10 Pg. 2605-8 (Oct 1991) ISSN: 0014-2980 [Print] Germany
• PMID: 1915562 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Immunoglobulin M
• Receptors, Antigen, B-Cell
• Cyclosporine
• Calcium

Topics

• Animals
• B-Lymphocytes (cytology)
• Calcium (physiology)
• Cell Division (drug effects)
• Cyclosporine (pharmacology)
• Immunoglobulin M (physiology)
• In Vitro Techniques
• Lymphoma, B-Cell (pathology)
• Mice
• Receptors, Antigen, B-Cell (physiology)
• Signal Transduction
• Tumor Cells, Cultured