Amelioration of murine dextran sulfate sodium-induced colitis by nuclear factor-kappaB decoy oligonucleotides.

Abstract

BACKGROUND:

Activation of nuclear factor (NF)-kappaB has been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). The purpose of the current study was to investigate the effects of NF-kappaB decoy oligonucleotides (ODNs) on an experimental model of UC.

METHODS:

NF-kappaB decoy ODNs were administered in experimental colitis induced by dextran sulfate sodium (DSS). The disease activity index (DAI) and histological score were observed. NF-kappaB DNA binding activity was assessed by electrophoretic mobility shift assay (EMSA). The expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA).

RESULTS:

A significant improvement was observed in DAI and histological score in mice with NF-kappaB decoy ODNs, and the increase in NF-kappaB DNA binding activity, myeloperoxidase (MPO) activity, IL-1beta, and TNF-a in mice with DSS-induced colitis was significantly reduced following administration of NF-kappaB decoy ODNs.

CONCLUSIONS:

The administration of NF-kappaB decoy ODNs leads to an amelioration of DSS-induced colitis, suggesting administration of NF-kappaB decoy ODNs may provide a therapeutic approach for UC.

• Authors: Jun Ying Xiang, Li Guo Wu, Xiao Li Huang, Meng Zhang, Lan Pen, Qin Ouyan, Hua Tian Gan
• Journal: American journal of surgery (Am J Surg) Vol. 197 Issue 6 Pg. 797-805 (Jun 2009) ISSN: 1879-1883 [Electronic] United States
• PMID: 18926515 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NF-kappaB decoy
• Oligodeoxyribonucleotides
• Dextran Sulfate

Topics

• Animals
• Colitis, Ulcerative (chemically induced, drug therapy)
• Dextran Sulfate (administration & dosage)
• Female
• Mice
• Mice, Inbred BALB C
• Oligodeoxyribonucleotides (therapeutic use)