Abstract | BACKGROUND: METHODS: Rats with intrathecal catheters were anesthetized and underwent plantar incision. Spontaneous pain behavior and withdrawal threshold to punctuate stimulation were measured before and after administration of intrathecal R-phenylisopropyl- adenosine ( R-PIA; A1R agonist), 2-w p-2-carbonyl-ethyl-phenylethylaminox-5X-N-ethylcarboxami-doadenosine ( CGS21680; A2aR agonist), or vehicle. In separate groups of animals, the effects of pertussis toxin, forskolin, glibenclamide, 4-aminopyridine, tetraethylammonium, apamin, charybdotoxin, or margatoxin on R-PIA-induced antinociception were examined. RESULTS: CONCLUSIONS: Spinal A1Rs but not A2aRs play an important role in the maintenance of nonevoked and evoked pain behaviors after an incision. Furthermore, A1R-induced spinal antinociception is mediated by interactions with pertussis toxin-sensitive G proteins. In addition, the opening of adenosine triphosphate-sensitive K channels but not of calcium-activated potassium channels and voltage-gated Kv1.3 or Kv1.6 channels contribute to the antinociceptive effect of A1R agonists.
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Authors | Peter K Zahn, Heidrun Straub, Manuel Wenk, Esther M Pogatzki-Zahn |
Journal | Anesthesiology
(Anesthesiology)
Vol. 107
Issue 5
Pg. 797-806
(Nov 2007)
ISSN: 0003-3022 [Print] United States |
PMID | 18073555
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adenosine A1 Receptor Agonists
- Adenosine A2 Receptor Agonists
- Potassium Channel Blockers
- Potassium Channels
- Vasodilator Agents
- Phenylisopropyladenosine
- Adenosine-5'-(N-ethylcarboxamide)
- Pertussis Toxin
- GTP-Binding Proteins
- Adenosine
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Topics |
- Adenosine
(administration & dosage, analogs & derivatives, pharmacology)
- Adenosine A1 Receptor Agonists
- Adenosine A2 Receptor Agonists
- Adenosine-5'-(N-ethylcarboxamide)
(administration & dosage, pharmacology)
- Animals
- Disease Models, Animal
- Dose-Response Relationship, Drug
- GTP-Binding Proteins
(drug effects, metabolism)
- Hyperalgesia
(drug therapy, etiology)
- Male
- Pain, Postoperative
(drug therapy)
- Pertussis Toxin
(pharmacology)
- Phenylisopropyladenosine
(administration & dosage, pharmacology)
- Physical Stimulation
- Potassium Channel Blockers
(administration & dosage, pharmacology)
- Potassium Channels
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Surgical Procedures, Operative
(adverse effects)
- Vasodilator Agents
(administration & dosage, pharmacology)
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