Antinociceptive and anti-inflammatory effects of compounds isolated from Scaphyglottis livida and Maxillaria densa.

Abstract

Oral administration of a CH(2)Cl(2)-MeOH (1:1) extract of Scaphyglottis livida produced dose-dependent antinociceptive and anti-inflammatory effects when tested in mice and rats using the hot-plate (150-600 mg/kg) and carrageenan-induced inflammation (150-600 mg/kg) models, respectively. Morphine (1.5-6 mg/kg, p.o.) and indomethacin (10-40 mg/kg, p.o.) were used as positive controls, respectively. Four compounds were isolated from the active extract of Scaphyglottis livida, namely 5alpha-lanosta-24,24-dimethyl-9(11),25-dien-3beta-ol (LDD), 24,24,dimethyl-9,19-cyclolanosta-9(11),25-dien-3-one (cyclobalanone), gigantol and 3,4'-dihydroxy-3',4,5-trimethoxybibenzyl (DTB). LDD and gigantol (25-100 mg/kg, p.o.) significantly increased the hot-plate latency in comparison to vehicle-treated mice and decreased carrageenan-induced inflammation in rats. The antinociception provoked by LDD and gigantol was partially blocked by naloxone (1mg/kg, i.p.). However, pretreatment with L-NAME (100 mg/kg, i.p.) and glibenclamide (10 mg/kg, i.p.) did not affect the antinociceptive response induced by LDD or gigantol suggesting that their pharmacological effect could be partially due to activation of opioid receptors. Moreover, a CH(2)Cl(2)-MeOH (1:1) extract of Maxillaria densa reduced acetic acid-induced abdominal writhes but was not able to produce antinociception in the hot-plate assay. Two compounds were isolated from the active extract of Maxillaria densa, namely fimbriol A and erianthridin. Both compounds partially reduced acetic acid-induced writhes. The results tend to support the popular use of this species in folk medicine for treatment of painful complaints.

• Authors: Myrna Déciga-Campos, Juan Francisco Palacios-Espinosa, Adelfo Reyes-Ramírez, Rachel Mata
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 114 Issue 2 Pg. 161-8 (Nov 01 2007) ISSN: 0378-8741 [Print] Ireland
• PMID: 17855030 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics
• Anti-Inflammatory Agents, Non-Steroidal
• Bibenzyls
• Plant Extracts
• gigantol
• Guaiacol
• Carrageenan
• Acetic Acid

Topics

• Acetic Acid
• Analgesics (isolation & purification, pharmacology)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (isolation & purification, pharmacology)
• Behavior, Animal (drug effects)
• Bibenzyls
• Carrageenan
• Chromatography, High Pressure Liquid
• Edema (chemically induced, prevention & control)
• Guaiacol (analogs & derivatives, chemical synthesis, isolation & purification, pharmacology)
• Magnetic Resonance Spectroscopy
• Male
• Mexico
• Mice
• Mice, Inbred ICR
• Orchidaceae (chemistry)
• Pain Measurement (drug effects)
• Plant Extracts (chemistry, pharmacology, toxicity)
• Rats
• Rats, Sprague-Dawley
• Reaction Time (drug effects)
• Spectrometry, Mass, Electrospray Ionization
• Spectrophotometry, Infrared