A TAF4-homology domain from the corepressor ETO is a docking platform for positive and negative regulators of transcription.

Abstract	The eight twenty-one protein, ETO, is implicated in 12%-15% of acute human leukemias as part of a gene fusion with RUNX1 (also called AML1). Of the four ETO domains related to Drosophila melanogaster Nervy, only two are required to induce spontaneous myeloid leukemia upon transplantation into the mouse. One of these domains is related in sequence to TAF4, a component of TFIID. The structure of this domain, ETO-TAFH, is similar to yeast Rpb4 and to Escherichia coli sigma(70); it is the first TAF-related protein with structural similarity to the multisubunit RNA polymerases. Overlapping surfaces of ETO-TAFH interact with an autonomous repression domain of the nuclear receptor corepressor N-CoR and with a conserved activation domain from the E-box family of transcription factors. Thus, ETO-TAFH acts as a structural platform that can interchange negative and positive coregulatory proteins to control transcription.
Authors	Yufeng Wei, Shaohua Liu, Jörn Lausen, Christopher Woodrell, Seongeun Cho, Nikolaos Biris, Naohiro Kobayashi, Yu Wei, Shigeyuki Yokoyama, Milton H Werner
Journal	Nature structural & molecular biology (Nat Struct Mol Biol) Vol. 14 Issue 7 Pg. 653-61 (Jul 2007) ISSN: 1545-9993 [Print] United States
PMID	17572682 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	DNA-Binding Proteins Proto-Oncogene Proteins RUNX1 Translocation Partner 1 Protein RUNX1T1 protein, human Repressor Proteins Transcription Factor TFIID Transcription Factors
Topics	Animals DNA-Binding Proteins (chemistry, genetics) Down-Regulation E-Box Elements Gene Expression Regulation Helix-Loop-Helix Motifs Humans Mice Molecular Sequence Data Protein Structure, Tertiary Proto-Oncogene Proteins (chemistry, genetics) RUNX1 Translocation Partner 1 Protein Repressor Proteins (chemistry, genetics) Sequence Homology, Amino Acid Transcription Factor TFIID (chemistry, genetics) Transcription Factors (chemistry, genetics) Transcription, Genetic Two-Hybrid System Techniques Up-Regulation

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